Controlling the polarity of human gastrointestinal organoids to investigate epithelial biology and infectious diseases

Julia Y. Co, Mar Margalef-Català, Denise M. Monack, Manuel R. Amieva

Published: 2021-10-17 DOI: 10.1038/s41596-021-00607-0

organoids

epithelial polarity

host-microbe interactions

gastrointestinal health

disease research

AI 解读

Author Correction: Full-length sequencing of circular DNA viruses and extrachromosomal circular DNA using CIDER-Seq

Generation of heart-forming organoids from human pluripotent stem cells

Design and fabrication of wearable electronic textiles using twisted fiber-based threads

Activation of mechanoluminescent nanotransducers by focused ultrasound enables light delivery to deep-seated tissue in vivo

Chromatin accessibility profiling by ATAC-seq

Deoxyfluorination of phenols for chemoselective18F-labeling of peptides

Author Correction: cfSNV: a software tool for the sensitive detection of somatic mutations from cell-free DNA

Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids

An inexpensive semi-automated sample processing pipeline for cell-free RNA extraction

Application of CHyMErA Cas9-Cas12a combinatorial genome-editing platform for genetic interaction mapping and gene fragment deletion screening

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Human epithelial organoids—3D spheroids derived from adult tissue stem cells—enable investigation of epithelial physiology and disease and host interactions with microorganisms, viruses and bioactive molecules. One challenge in using organoids is the diff
Introduction
Materials
Procedure
- Apical-out GI epithelial organoids in suspension culture
- Downstream assays
Troubleshooting
Timing
Anticipated results
- References
References
ACKNOWLEDGMENTS

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Controlling the polarity of human gastrointestinal organoids to investigate epithelial biology and infectious diseases

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