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换一换Total and PS129 aSyn levels using Western Blot
daniel.dautan daniel, Wojciech Paslawski, Per Svenningsson
Measure total and ps129 alpha-synuclein in mouse plasma.
AI 解读Poly-ornithine/laminin substrate for neural cell culture
Gist Croft, Regine Tipon
This protocol is used to create adhesive and bioactive substrate for neural cell types, low to high density nuerons, astrocytes, or organoids. It is based on standard methods but includes several optimizations, use case recommendations, and alternatives, and advice.
AI 解读Quick and simple removal of endotoxins from purified protein solutions with magnetic beads
Gudrun Baersch
Endotoxins (lipopolysaccharides) can affect the results of protein solutions in many ways, so it is important to eliminate them before in vitro and in vivo testing. There are several resin and chromatography- based solutions on the market. However, the use of these commercially available endotoxin elimination methods leads to significant losses of protein. E. coli produces endotoxins as it is utilized to express a variety of commercially accessible recombinant proteins. The presence of trace amounts of endotoxins has negative effects on proteinsolutions. Therefore, we have developed magnetic beads that eliminate such endotoxins when introduced into solutions. There is no loss of proteins in these applications. Magnetic beads offer a simple and fast way to remove these endotoxins. They are also cost-effective because the beads used can be regenerated and reused multiple times.
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