Efficient and safe single-cell cloning of human pluripotent stem cells using the CEPT cocktail

Carlos A. Tristan, Hyenjong Hong, Yogita Jethmalani, Yu Chen, Claire Weber, Pei-Hsuan Chu, Seungmi Ryu, Vukasin M. Jovanovic, Inae Hur, Ty C. Voss, Anton Simeonov, Ilyas Singeç

Published: 2022-10-18 DOI: 10.1038/s41596-022-00753-z

microfluidics

hPSCs

single-cell cloning

gene editing

CEPT cocktail

AI 解读

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Human pluripotent stem cells (hPSCs) are inherently sensitive cells. Single-cell dissociation and the establishment of clonal cell lines have been long-standing challenges. This inefficiency of cell cloning represents a major obstacle for the standardizat
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
Limitations
- Reporting summary
- References
References
ACKNOWLEDGMENTS
ACKNOWLEDGMENTS

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Efficient and safe single-cell cloning of human pluripotent stem cells using the CEPT cocktail

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