Protocols and risks: when less is more Jaspreet Pannu, Jonas B. Sandbrink, Matthew Watson, Megan J. Palmer, David A. Relman Published: 2021-12-05 DOI: 10.1038/s41596-021-00655-6 AI 解读 浏览 1163 PDFMetadata 暂无数据 推荐阅读 Nature Protocols Protocols IO Current Protocols Hyperspectral confocal imaging for high-throughput readout and analysis of bio-integrated microlasers Combinatorial fluorescent labeling of live anaerobic bacteria via the incorporation of azide-modified sugars into newly synthesized macromolecules Three-dimensional human neural culture on a chip recapitulating neuroinflammation and neurodegeneration A plate-based single-cell ATAC-seq workflow for fast and robust profiling of chromatin accessibility Reproducible mass spectrometry data processing and compound annotation in MZmine 3 Production and use of antigen tetramers to study antigen-specific B cells Probing the free-state solution behavior of drugs and their tendencies to self-aggregate into nano-entities Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning Design of metal-mediated protein assemblies via hydroxamic acid functionalities Preparation of AIEgen-based near-infrared afterglow luminescence nanoprobes for tumor imaging and image-guided tumor resection 查看全部 Electroporation of fluorescent antisense molecules into Bodo saltans and live imaging Standard operating procedure for the isolation of genetically engineered hPSCs clones in a high-throughput way ASAPCRN iNDI Transcription Factor-NGN2 differentiation of human iPSC into cortical neurons Version 2 Obtain LB broth medium FLOUR-seq Cryptococcus neoformans DNA Extraction Method Passive avoidance (step-down test) U54 SCENT T/NK Immunosenescence Profiling Flow Cytometry Panel Workflow for wooden contact samples in use-wear experiments with bronze axe replicas (MAP-protocol_B) archaeology use-wear traceology bronze axes socketed axes contact materials contact samples experiment experimental archaeology bronze age beechwood controlled experiment Enriched Rat Housing 查看全部 Exploring Chemical Information in PubChem cheminformatics chemical structure search drug discovery molecular similarity PubChem public database Analysis of Globotriaosylceramide (Gb3) in Liquid Urine: A Straightforward Assay Using Tandem Mass Spectrometry creatinine Fabry disease Gb3 globotriaosylceramide mass spectrometry UHPLC-MS/MS Software Tool for Automatic Quantification of Sarcomere Length and Organization in Fixed and Live 2D and 3D Muscle Cell Cultures In Vitro cardiomyocytes sarcomere length sarcomere organization skeletal muscle software tool stem cells In Vitro Assessment of Cardiac Fibroblast Activation at Physiologic Stiffness activation cardiac fibroblasts cell culture fibrosis myofibroblast PDMS TGFβ Differentiation, Transcriptomic Profiling, and Calcium Imaging of Human Hypothalamic Neurons differentiation human hypothalamus neuron obesity pluripotent stem cell POMC protocol Optimized Recombinant Expression and Purification of the SARS-CoV-2 Polymerase Complex baculovirus E. coli SARS-CoV-2 viral polymerase complex Loading Determination of DMTr-substituted Resins for Large-scale Oligonucleotide Synthesis DMTr-substituted resins DMTr determination oligonucleotide synthesis substitution of resins SPOS solid-phase oligonucleotide synthesis UV/vis spectroscopy Antibody-Based CAR T Cells Produced by Lentiviral Transduction CAR T cells chimeric antigen receptor lentiviral transduction Isolation, Culture, and Transfection of Melanocytes epidermis keratinocytes melanocytes primary culture transfection The Isolation of Stress Granules From Plant Material affinity purification Arabidopsis thaliana metabolome plant proteome stress granules stress response 查看全部
