Preparing ductal epithelial organoids for high-spatial-resolution molecular profiling using mass spectrometry imaging

Brenda Bakker, Rianne D. W. Vaes, Merel R. Aberle, Tessa Welbers, Thomas Hankemeier, Sander S. Rensen, Steven W. M. Olde Damink, Ron M. A. Heeren

Published: 2022-02-17 DOI: 10.1038/s41596-021-00661-8

organoids

mass spectrometry imaging

3D morphological structure

gelatin embedding

molecular profiling

AI 解读

Supplementary information

Supplementary Information

Supplementary Methods 1–6, Supplementary Figs. 1–4 and Supplementary Table 1.

Reporting Summary

Purification of infection-associated macropinosomes by magnetic isolation for proteomic characterization

Hi-CO: 3D genome structure analysis with nucleosome resolution

Practical and concise synthesis of nucleoside analogs

Comprehensive analysis of glycosphingolipid glycans by lectin microarrays and MALDI-TOF mass spectrometry

Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids

KAS-seq: genome-wide sequencing of single-stranded DNA by N3-kethoxal–assisted labeling

Detecting chromosomal interactions in Capture Hi-C data with CHiCAGO and companion tools

Capture of the newly transcribed RNA interactome using click chemistry

Design, assembly, and characterization of membrane-spanning DNA nanopores

Transcriptome-wide identification of RNA-binding protein binding sites using seCLIP-seq

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References

Supplementary information

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Preparing ductal epithelial organoids for high-spatial-resolution molecular profiling using mass spectrometry imaging

Supplementary information

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