Off-the-shelf proximity biotinylation using ProtA-TurboID

Irene Santos-Barriopedro, Guido van Mierlo, Michiel Vermeulen

Published: 2022-10-11 DOI: 10.1038/s41596-022-00748-w

Proteomics

Mass spectrometry

Proximity biotinylation

TurboID

Protein A

AI 解读

Purification of infection-associated macropinosomes by magnetic isolation for proteomic characterization

Hi-CO: 3D genome structure analysis with nucleosome resolution

Practical and concise synthesis of nucleoside analogs

Comprehensive analysis of glycosphingolipid glycans by lectin microarrays and MALDI-TOF mass spectrometry

Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids

KAS-seq: genome-wide sequencing of single-stranded DNA by N3-kethoxal–assisted labeling

Detecting chromosomal interactions in Capture Hi-C data with CHiCAGO and companion tools

Capture of the newly transcribed RNA interactome using click chemistry

Design, assembly, and characterization of membrane-spanning DNA nanopores

Transcriptome-wide identification of RNA-binding protein binding sites using seCLIP-seq

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References

Proximity biotinylation is a commonly used method to identify the invivo proximal proteome for proteins of interest. This technology typically relies on fusing a bait protein to a biotin ligase using overexpression or clustered regularly interspaced shor
Introduction
Materials
Procedure
Troubleshooting
Anticipated results
Timing
- Reporting summary
- References
References
ACKNOWLEDGMENTS

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Off-the-shelf proximity biotinylation using ProtA-TurboID

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