Mass cytometry profiling of human dendritic cells in blood and tissues
Marcela Alcántara-Hernández, Juliana Idoyaga



























Extended
Extended Data Fig. 1 Frequencies of leukocytes in blood and skin samples before and after enrichment.
a , PBMCs were obtained from blood (Steps 1–10). b , Skin cell suspensions were obtained after enzymatic digestion (Steps 23–37). Myeloid cells or leukocytes were negatively enriched from blood and skin, respectively, following the procedures described in Steps 45–53. Cells were stained for flow cytometry before and after negative enrichment. One representative blood and skin sample is shown. Numbers represent frequency in the gate. Antibodies used are as follows: CD45 Brilliant Violet 785 (Biolegend, cat. no. 304049), CD3 PerCPCy5.5 (Biolegend, cat. no. 300430), CD19 PerCPCy5.5 (Biolegend, cat. no. 302230), CD335 PerCPCy5.5 (Biolegend, cat. no. 331920), CD66b PerCPCy5.5 (Biolegend, cat. no. 305108). Figure created for this protocol from data obtained as part of a primary publication 5 .