Infection of zebrafish larvae with human norovirus and evaluation of the in vivo efficacy of small-molecule inhibitors

Jana Van Dycke, Arno Cuvry, Jan Knickmann, Annelii Ny, Sebastian Rakers, Stefan Taube, Peter de Witte, Johan Neyts, Joana Rocha-Pereira

Published: 2021-04-08 DOI: 10.1038/s41596-021-00499-0

zebrafish larvae

human norovirus

viral replication

small-molecule inhibitors

antiviral efficacy

AI 解读

Author Correction: Full-length sequencing of circular DNA viruses and extrachromosomal circular DNA using CIDER-Seq

Generation of heart-forming organoids from human pluripotent stem cells

Design and fabrication of wearable electronic textiles using twisted fiber-based threads

Activation of mechanoluminescent nanotransducers by focused ultrasound enables light delivery to deep-seated tissue in vivo

Chromatin accessibility profiling by ATAC-seq

Deoxyfluorination of phenols for chemoselective18F-labeling of peptides

Author Correction: cfSNV: a software tool for the sensitive detection of somatic mutations from cell-free DNA

Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids

An inexpensive semi-automated sample processing pipeline for cell-free RNA extraction

Application of CHyMErA Cas9-Cas12a combinatorial genome-editing platform for genetic interaction mapping and gene fragment deletion screening

查看全部

Sections

Figures

References

We have recently established that human norovirus (HuNoV) replicates efficiently in zebrafish larvae after inoculation of a clinical sample into the yolk, providing a simple and robust invivo system in which to study HuNoV. In this Protocol Extension, we
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
- Reporting Summary
- References
References
ACKNOWLEDGMENTS

Cheetham, S. et al. Pathogenesis of a genogroup II human norovirus in gnotobiotic pigs. J. Virol. 80, 10372–10381 (2006).
Bartsch, S. M., Lopman, B. A., Ozawa, S., Hall, A. J. & Lee, B. Y. Global economic burden of norovirus gastroenteritis. PloS ONE 11, e0151219 (2016).
Jones, M. K. et al. Enteric bacteria promote human and mouse norovirus infection of B cells. Science 346, 755–759 (2014).
Ettayebi, K. et al. Replication of human noroviruses in stem cell-derived human enteroids. Science 353, 1387–1393 (2016).
Van Dycke, J. et al. A robust human norovirus replication model in zebrafish larvae. PLoS Pathog. 15, e1008009 (2019).
Souza, M., Azevedo, M. S., Jung, K., Cheetham, S. & Saif, L. J. Pathogenesis and immune responses in gnotobiotic calves after infection with the genogroup II.4-HS66 strain of human norovirus. J. Virol. 82, 1777–1786 (2008).
Taube, S. et al. A mouse model for human norovirus. mBio https://doi.org/10.1128/mBio.00450-13 (2013).
Sullivan, C. et al. Using zebrafish models of human influenza A virus infections to screen antiviral drugs and characterize host immune cell responses. J. Vis. Exp. https://doi.org/10.3791/55235 (2017).
Harvie, E. A. & Huttenlocher, A. Non-invasive imaging of the innate immune response in a zebrafish larval model of Streptococcus iniae infection. J. Vis. Exp. https://doi.org/10.3791/52788 (2015).
Mazon Moya, M. J., Colucci-Guyon, E. & Mostowy, S. Use of Shigella flexneri to study autophagy-cytoskeleton interactions. J. Vis. Exp. https://doi.org/10.3791/51601 (2014).
Howe, K. et al. The zebrafish reference genome sequence and its relationship to the human genome. Nature 496, 498–503 (2013).
Aleström, P. et al. Zebrafish: housing and husbandry recommendations. Lab Anim. https://doi.org/10.1177/0023677219869037 (2019).
Costantini, V. et al. Human norovirus replication in human intestinal enteroids as model to evaluate virus inactivation. Emerg. Infect. Dis. 24, 1453–1464 (2018).
Takaki, K., Davis, J. M., Winglee, K. & Ramakrishnan, L. Evaluation of the pathogenesis and treatment of Mycobacterium marinum infection in zebrafish. Nat. Protoc. 8, 1114–1124 (2013).
Saydmohammed, M. & Tsang, M. High-throughput automated chemical screens in zebrafish. Methods Mol. Biol. (Clifton, N.J.) 1683, 383–393 (2018).
Gray, C. et al. Simultaneous intravital imaging of macrophage and neutrophil behaviour during inflammation using a novel transgenic zebrafish. Thromb. Haemost. 105, 811–819 (2011).
Willis, A. R. et al. Shigella-induced emergency granulopoiesis protects zebrafish larvae from secondary infection. mBio 9, e00933–00918 (2018).
Miskolci, V. et al. Distinct inflammatory and wound healing responses to complex caudal fin injuries of larval zebrafish. eLife 8, e45976 (2019).
Burgos, J. S., Ripoll-Gomez, J., Alfaro, J. M., Sastre, I. & Valdivieso, F. Zebrafish as a new model for herpes simplex virus type 1 infection. Zebrafish 5, 323–333 (2008).
Gabor, K. A. et al. Influenza A virus infection in zebrafish recapitulates mammalian infection and sensitivity to anti-influenza drug treatment. Dis. Model. Mech. 7, 1227–1237 (2014).
Palha, N. et al. Real-time whole-body visualization of Chikungunya virus infection and host interferon response in zebrafish. PLoS Pathog. 9, e1003619 (2013).
Wilson, C. Aspects of larval rearing. ILAR J. 53, 169–178 (2012).
Gore, A. V., Pillay, L. M., Venero Galanternik, M. & Weinstein, B. M. The zebrafish: a fintastic model for hematopoietic development and disease. Wiley Interdiscip. Rev. Dev. Biol. 7, e312–e312 (2018).
Goody, M. F., Sullivan, C. & Kim, C. H. Studying the immune response to human viral infections using zebrafish. Dev. Comp. Immunol. 46, 84–95 (2014).
Kanther, M. & Rawls, J. F. Host-microbe interactions in the developing zebrafish. Curr. Opin.Immunol. 22, 10–19 (2010).
Lewis, K. L., Del Cid, N. & Traver, D. Perspectives on antigen presenting cells in zebrafish. Dev. Comp. Immunol. 46, 63–73 (2014).
Lister, J. A., Robertson, C. P., Lepage, T., Johnson, S. L. & Raible, D. W. nacre encodes a zebrafish microphthalmia-related protein that regulates neural-crest-derived pigment cell fate. Development 126, 3757–3767 (1999).
Karlsson, J., von Hofsten, J. & Olsson, P. E. Generating transparent zebrafish: a refined method to improve detection of gene expression during embryonic development. Mar. Biotechnol. 3, 522–527 (2001).
Stals, A. et al. Multiplex real-time RT-PCR for simultaneous detection of GI/GII noroviruses and murine norovirus 1. J. Virol. Methods 161, 247–253 (2009).
Kageyama, T. et al. Broadly reactive and highly sensitive assay for Norwalk-like viruses based on real-time quantitative reverse transcription-PCR. J. Clin. Microbiol. 41, 1548–1557 (2003).
Balla, K. M., Rice, M. C., Gagnon, J. A. & Elde, N. C. Linking virus discovery to immune responses visualized during zebrafish infections. Curr. Biol. 30, 2092–2103.e2095 (2020).
Giusti, A. et al. Safety assessment of compounds after in vitro metabolic conversion using zebrafish eleuthero embryos. Int. J. Mol. Sci. https://doi.org/10.3390/ijms20071712 (2019).

Infection of zebrafish larvae with human norovirus and evaluation of the in vivo efficacy of small-molecule inhibitors

Supplementary information

推荐阅读