Induction of human trophoblast stem cells

Gaël Castel, Laurent David

Published: 2022-10-13 DOI: 10.1038/s41596-022-00744-0

induced trophoblast stem cells

placental development

cell reprogramming

genome-specific disease modeling

syncytiotrophoblast differentiation

AI 解读

Fabrication of angstrom-scale two-dimensional channels for mass transport

Efficient and strand-specific profiling of replicating chromatin with enrichment and sequencing of protein-associated nascent DNA in mammalian cells

The eINTACT method for studying nuclear changes in host plant cells targeted by bacterial effectors in native infection contexts

Rapid reaction optimization by robust and economical quantitative benchtop19F NMR spectroscopy

Profiling native pulmonary basement membrane stiffness using atomic force microscopy

Photoimmunotechnology as a powerful biological tool for molecular-based elimination of target cells and microbes, including bacteria, fungi and viruses

Measuring key human carbohydrate digestive enzyme activities using high-performance anion-exchange chromatography with pulsed amperometric detection

Neural cell isolation from adult macaques for high-throughput analyses and neurosphere cultures

Preparation, validation and use of a vasoactive tryptophan-derived hydroperoxide and relevant control compounds

Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes

查看全部

Sections

Figures

References

Cell reprogramming has allowed unprecedented access to human development, from virtually any genome. However, reprogramming yields pluripotent stem cells that can differentiate into all cells that form a fetus, but not extraembryonic annexes. Therefore, a
Introduction
Materials
Procedure 1
- Somatic cell reprogramming of adult fibroblasts into induced TSCs
- Procedure
Procedure 2
- Conversion of naive/extended pluripotent stem cells into TSCs
- Procedure
Procedure 3
- Differentiation of hi/cTSCs into ST and EVT
- Culture on fibronectin
Troubleshooting
Timing
Anticipated results
References
ACKNOWLEDGMENTS

Okae, H. et al. Derivation of human trophoblast stem cells. Cell Stem Cell 22, 50–63.e6 (2018).
Haider, S. et al. Self-renewing trophoblast organoids recapitulate the developmental program of the early human placenta. Stem Cell Rep. 11, 537–551 (2018).
Turco, M. Y. et al. Trophoblast organoids as a model for maternal-fetal interactions during human placentation. Nature 564, 263–267 (2018).
Saha, B. et al. TEAD4 ensures postimplantation development by promoting trophoblast self-renewal: an implication in early human pregnancy loss. Proc. Natl Acad. Sci. USA 117, 17864–17875 (2020).
Castel, G. et al. Induction of human trophoblast stem cells from somatic cells and pluripotent stem cells. Cell Rep. 33, 108419 (2020).
Amita, M. et al. Complete and unidirectional conversion of human embryonic stem cells to trophoblast by BMP4. Proc. Natl Acad. Sci. USA 110, E1212–E1221 (2013).
Krendl, C. et al. GATA2/3-TFAP2A/C transcription factor network couples human pluripotent stem cell differentiation to trophectoderm with repression of pluripotency. Proc. Natl Acad. Sci. USA 114, E9579–E9588 (2017).
Horii, M. et al. Human pluripotent stem cells as a model of trophoblast differentiation in both normal development and disease. Proc. Natl Acad. Sci. USA 113, E3882–E3891 (2016).
Horii, M. et al. Modeling preeclampsia using human induced pluripotent stem cells. Sci. Rep. 11, 5877 (2021).
Sheridan, M. A. et al. Early onset preeclampsia in a model for human placental trophoblast. Proc. Natl Acad. Sci. USA 116, 4336–4345 (2019).
Takashima, Y. et al. Resetting transcription factor control circuitry toward ground-state pluripotency in human. Cell 158, 1254–1269 (2014).
Yang, Y. et al. Derivation of pluripotent stem cells with in vivo embryonic and extraembryonic potency. Cell 169, 243–257.e25 (2017).
Amit, M. et al. Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Dev. Biol. 227, 271–278 (2000).
Rossant, J. & Tam, P. P. L. Opportunities and challenges with stem cell-based embryo models. Stem Cell Rep. 16, 1031–1038 (2021).
Zhou, J. et al. Modeling human peri-implantation placental development and function. Biol. Reprod. 105, 40–51 (2021).
Horii, M., Touma, O., Bui, T. & Parast, M. M. Modeling human trophoblast, the placental epithelium at the maternal fetal interface. Reproduction 160, R1–R11 (2020).
Moser, G., Weiss, G., Gauster, M., Sundl, M. & Huppertz, B. Evidence from the very beginning: endoglandular trophoblasts penetrate and replace uterine glands in situ and in vitro. Hum. Reprod. 30, 2747–2757 (2015).
Moser, G. et al. Extravillous trophoblasts invade more than uterine arteries: evidence for the invasion of uterine veins. Histochem. Cell Biol. 147, 353–366 (2017).
Moser, G., Drewlo, S., Huppertz, B. & Armant, D. R. Trophoblast retrieval and isolation from the cervix: origins of cervical trophoblasts and their potential value for risk assessment of ongoing pregnancies. Hum. Reprod. Update 24, 484–496 (2018).
Papuchova, H. et al. Three types of HLA-G+ extravillous trophoblasts that have distinct immune regulatory properties. Proc. Natl Acad. Sci. USA 117, 15772–15777 (2020).
Pollheimer, J., Vondra, S., Baltayeva, J., Beristain, A. G. & Knöfler, M. Regulation of placental extravillous trophoblasts by the maternal uterine environment. Front. Immunol. 9, 2597 (2018).
Borbely, A. U. et al. The term basal plate of the human placenta as a source of functional extravillous trophoblast cells. Reprod. Biol. Endocrinol. 12, 7 (2014).
Dong, C. et al. Derivation of trophoblast stem cells from naïve human pluripotent stem cells. Elife 9, e52504 (2020).
Genbacev, O., Joslin, R., Damsky, C. H., Polliotti, B. M. & Fisher, S. J. Hypoxia alters early gestation human cytotrophoblast differentiation/invasion in vitro and models the placental defects that occur in preeclampsia. J. Clin. Invest. 97, 540–550 (1996).
Liu, X. et al. Reprogramming roadmap reveals route to human induced trophoblast stem cells. Nature 586, 101–107 (2020).
Tan, J. P., Liu, X. & Polo, J. M. Establishment of human induced trophoblast stem cells via reprogramming of fibroblasts Nat. Protoc. in press.
Cinkornpumin, J. K. et al. Naive human embryonic stem cells can give rise to cells with a trophoblast-like transcriptome and methylome. Stem Cell Rep. 15, 198–213 (2020).
Guo, G. et al. Human naive epiblast cells possess unrestricted lineage potential. Cell Stem Cell 28, 1040–1056.e6 (2021).
Gao, X. et al. Establishment of porcine and human expanded potential stem cells. Nat. Cell Biol. 21, 687–699 (2019).
Kagawa, H. et al. Human blastoids model blastocyst development and implantation. Nature 601, 600–605 (2022).
Liu, X. et al. Modelling human blastocysts by reprogramming fibroblasts into iBlastoids. Nature 591, 627–632 (2021).
Sozen, B. et al. Reconstructing aspects of human embryogenesis with pluripotent stem cells. Nat. Commun. 12, 5550 (2021).
Yanagida, A. et al. Naive stem cell blastocyst model captures human embryo lineage segregation. Cell Stem Cell 28, 1016–1022.e4 (2021).
Yu, L. et al. Blastocyst-like structures generated from human pluripotent stem cells. Nature 591, 620–626 (2021).
Kudo, Y. et al. Quantifying the syncytialisation of human placental trophoblast BeWo cells grown in vitro. Biochim. Biophys. Acta 1640, 25–31 (2003).
Wice, B., Menton, D., Geuze, H. & Schwartz, A. L. Modulators of cyclic AMP metabolism induce syncytiotrophoblast formation in vitro. Exp. Cell Res. 186, 306–316 (1990).
David, L. & Polo, J. M. Phases of reprogramming. Stem Cell Res. 12, 754–761 (2014).
Takahashi, S. et al. Loss of p57KIP2 expression confers resistance to contact inhibition in human androgenetic trophoblast stem cells. Proc. Natl Acad. Sci. USA 116, 26606–26613 (2019).
Jozefczuk, J., Drews, K. & Adjaye, J. Preparation of mouse embryonic fibroblast cells suitable for culturing human embryonic and induced pluripotent stem cells. J. Vis. Exp. 2012, 3854 (2012).
Kilens, S. et al. Parallel derivation of isogenic human primed and naive induced pluripotent stem cells. Nat. Commun. 9, 360 (2018).

Induction of human trophoblast stem cells

推荐阅读