In vitro reconstitution of COPII vesicles fromArabidopsis thalianasuspension-cultured cells Baiying Li, Yonglun Zeng, Sze Wan Lo, Yusong Guo, Liwen Jiang Published: 2023-01-03 DOI: 10.1038/s41596-022-00781-9 Arabidopsis thaliana COPII vesicles in vitro reconstitution plant microsomes vesicle purification AI 解读 浏览 1404 PDFMetadataSupplementary information Reporting Summary 推荐阅读 Nature Protocols Protocols IO Current Protocols Transplantation of intestinal organoids into a mouse model of colitis Cultivation of previously uncultured microorganisms with a continuous-flow down-flow hanging sponge (DHS) bioreactor, using a syntrophic archaeon culture obtained from deep marine sediment as a case study NovoSpaRc: flexible spatial reconstruction of single-cell gene expression with optimal transport Directed differentiation of human induced pluripotent stem cells to hepatic stellate cells Chromatin accessibility profiling by ATAC-seq Nuclei isolation of multiple brain cell types for omics interrogation Author Correction: Constructing a cost-efficient, high-throughput and high-quality single-molecule localization microscope for super-resolution imaging High-resolution, ultrasensitive and quantitative DNA double-strand break labeling in eukaryotic cells using i-BLESS Use of deep learning to develop continuous-risk models for adverse event prediction from electronic health records Universal platform for the generation of thermostabilized GPCRs that crystallize in LCP 查看全部 Digestion for BioBrick Assembly Kit (E0546) Biobrick Assembly E0546 GeoMx-NGS Readout Library Preparation OPestTL V1.4 -- the Open Pesticide Transition Library Pesticides LCMS LCMSMethods.org Johns Hopkins Pesticide Research mass spectrometry Generating rabbit rAbs from heterohybridomas Piggybac-mediated stable expression of NGN2 in iPSCs for differentiation into excitatory glutamatergic neurons iPSC Differentiation iNeuron Piggybac NGN2 ASAPCRN Tween 60-esculin Agar (TE slant) Freely moving recording: Chronic recoverable Neuropixels in mice electrophysiology silicon probe behavior position tracking linear track H-maze Arduino headfixed spatial navigation Sanger Tree of Life Wet Laboratory Protocol Collection reference genome long read sequencing sample preparation tissue dissection sample homogenisation PowerMasher cryoPREP tissue disruption cryogenic tissue disruption plant tissue disruption tissue homogenisation cryogenic bead beating HMW DNA extraction magnetic bead extraction automated DNA extraction KingFisher MagAttract manual DNA extraction plant HMW DNA extraction solid phase reversible immobilisation Nanobind nucleated blood extractions mollusc extraction organic extraction next generation sequencing third generation sequencing HMW DNA fragmentation Megaruptor low input PacBio ultra-low input Covaris g-TUBE SPRI automated SPRI manual SPRI AMPure PB beads DNA clean-up RNA extraction manual RNA extraction TRIzol RNA-Seq Iso-Seq automated RNA extraction MagMax mirVana magnetic beads HMW DNA pooling Monosynaptic Rabies Tracing Mouse Surgery Stereotaxic Surgery Implants ASAPCRN Intracellular cytokine detection based on flow cytometry in hemocytes from Galleria mellonella larvae cytokine detection flow cytometry Galleria mellonella hemocytes 查看全部 Simple Workflow and Comparison of Media for hPSC-Cardiomyocyte Cryopreservation and Recovery cardiomyocytes cryopreservation freezing media functional recovery human pluripotent stem cells Modulating Myogenesis: An Optimized In Vitro Assay to Pharmacologically Influence Primary Myoblast Differentiation in vitro differentiation metformin myogenesis pharmacological modulation primary myoblast Using ConSurf to Detect Functionally Important Regions in RNA ConSurf evolutionary analysis functional regions Rate4Site RNA sequence analysis Streptozotocin-Induced Diabetic Models in Mice and Rats high-fat hyperglycemia insulin deficiency insulin resistance insulitis mouse or rat nicotinamide streptozotocin type 1 diabetes mellitus type 2 diabetes mellitus Fluorescent Protein Production, Purification, and Coupling to Microspheres fluorescent proteins full-spectrum cytometry microspheres protein expression protein purification From Hair to iPSCs—A Guide on How to Reprogram Keratinocytes and Why hair induced pluripotent stem cells (iPSCs) keratinocytes outer root sheath reprogramming Gnotobiotic Plant Systems for Reconstitution and Functional Studies of the Root Microbiota gnotobiotic reconstitution innate immunity phytostrip root microbiota Rapid Tagging of Human Proteins with Fluorescent Reporters by Genome Engineering using Double-Stranded DNA Donors Cas9 CRISPR double-stranded DNA donors fluorescent protein genome engineering homology-directed repair tissue culture cells Identification and Quantification of Monosaccharides from Fungal Cell Walls and Exopolysaccharides by Gas Chromatography Coupled to Mass Spectrometry biofilm cell wall exopolysaccharide GC-MS monosaccharide An In Vitro Microneutralization Assay for Influenza Virus Serology influenza neutralization microneutralization hemagglutinin HA neuraminidase NA 查看全部