In vitro reconstitution of COPII vesicles fromArabidopsis thalianasuspension-cultured cells Baiying Li, Yonglun Zeng, Sze Wan Lo, Yusong Guo, Liwen Jiang Published: 2023-01-03 DOI: 10.1038/s41596-022-00781-9 Arabidopsis thaliana COPII vesicles in vitro reconstitution plant microsomes vesicle purification AI 解读 浏览 1935 PDFMetadataSupplementary information Reporting Summary 推荐阅读 Nature Protocols Protocols IO Current Protocols Generation of mouse–human chimeric embryos Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues Decoding personal biotic and abiotic airborne exposome A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst Neuroscience robotics for controlled induction and real-time assessment of hallucinations Scanorama: integrating large and diverse single-cell transcriptomic datasets Single-cell RNA sequencing (scRNA-seq)(单细胞RNA测序) Data integration(数据集成) Heterogeneity(异质性) 查看全部 Intrahepatic implantation of tumor cells Intrahepatic tumor implantation Differential transcript usage analysis Small volume fungal genomic DNA extraction protocol for Illumina genome BIDMC TMC - low input single nuclei sequencing isolation (NNLB) Preparation of 1M calcium chloride solution (CaCl2) ngm worm c.elegans c elegans elegans caenorhabditis media medium calcium chloride Gradient PCR with DMSO gDNA RNA Clean Up Protocol Processamento de actígrafos pré-coleta - ActTrust actigrafia sono cronobiologia condor instruments Multiplex PCR Assay for Clade-typing Salmonella Enteritidis Non-typhoidal Salmonella Real-time PCR Phylogeny Molecular surveillance Generating a low-copy overexpression cell line using PhiC31 integration phiC31 overexpression attb 查看全部 Optimized and Scalable Precoating-Free Reprogramming of Human Peripheral Blood Mononuclear Cells into iPSCs coating extracellular matrix induced pluripotent stem cells laminin-511 reprogramming PBMCs scaling down Methods to Detect Small Molecule Inhibition of RING E3 Ligase Activity alpha screen RING E3 ligase small molecules TRAF6 Ubc13 ubiquitination Reprogramming Urine-Derived Cells Using Commercially Available Self-Replicative RNA and a Single Electroporation induced pluripotent stem cell reprogramming self-replicative RNA urine Fluorescent Protein Production, Purification, and Coupling to Microspheres fluorescent proteins full-spectrum cytometry microspheres protein expression protein purification RNA Extraction from Plant Tissue with Homemade Acid Guanidinium Thiocyanate Phenol Chloroform (AGPC) AGPC home-made buffers molecular biology protocol multiple plant species and tissues nucleic acids RNA isolation Production and Thermal Exchange of Conditional Peptide-MHC I Multimers CD8+ T cell staining MHC class I multimer peptide thermal exchange A Step-by-Step Guide for the Production of Recombinant Fluorescent TAT-HA-Tagged Proteins and their Transduction into Mammalian Cells DNA molecular assembly fluorescent protein fusion protein Ni-NTA-based protein purification protein transduction recombinant protein production tagged protein Sperm Chromatin Structure Assay (SCSA®) for Fertility Assessment DNA integrity flow cytometry male fertility pregnancy outcomes SCSA sperm chromatin structure assay ntLink: A Toolkit for De Novo Genome Assembly Scaffolding and Mapping Using Long Reads de novo genome assembly long reads minimizer mapping scaffolding ntLink An Efficient Agrobacterium-Mediated Transformation Protocol for Hexaploid and Tetraploid Wheat Agrobacterium CRISPR genome editing immature embryo wheat transformation 查看全部