Hyperosmolar blood–brain barrier opening using intra-arterial injection of hyperosmotic mannitol in mice under real-time MRI guidance

Chengyan Chu, Anna Jablonska, Yue Gao, Xiaoyan Lan, Wojciech G. Lesniak, Yajie Liang, Guanshu Liu, Shen Li, Tim Magnus, Monica Pearl, Miroslaw Janowski, Piotr Walczak

Published: 2021-12-12 DOI: 10.1038/s41596-021-00634-x

Blood–brain barrier

Mannitol

Interventional MRI

Therapeutic delivery

Mouse model

AI 解读

Supplementary information

Supplementary Information

Supplementary Figs. 1–4 and Supplementary Methods.

Reporting Summary

Supplementary Data 1

Matlab code and a demonstration of image processing

Supplementary Video 1

Temporary ligation of ECA and OA cauterization

Supplementary Video 2

Temporary ligation of PPA

Supplementary Video 3

Catheter cannulation

Supplementary Video 4

Brain perfusion of a contrast agent under real-time MRI

Supplementary Video 5

Postoperative procedures

Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells

The cell-line-derived subcutaneous tumor model in preclinical cancer research

Subcellular patch-clamp techniques for single-bouton stimulation and simultaneous pre- and postsynaptic recording at cortical synapses

NovoSpaRc: flexible spatial reconstruction of single-cell gene expression with optimal transport

Genome-wide pooled CRISPR screening in neurospheres

High-throughput cultivation and identification of bacteria from the plant root microbiota

Viral crosslinking and solid-phase purification enables discovery of ribonucleoprotein complexes on incoming RNA virus genomes

Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility

Addendum: Micro-CT acquisition and image processing to track and characterize pulmonary nodules in mice

Multiplexed single-cell proteomics using SCoPE2

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References

Supplementary information

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Hyperosmolar blood–brain barrier opening using intra-arterial injection of hyperosmotic mannitol in mice under real-time MRI guidance

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