Human fetal whole-body postmortem microfocus computed tomographic imaging

Ian C. Simcock, Susan C. Shelmerdine, J. Ciaran Hutchinson, Neil J. Sebire, Owen J. Arthurs

Published: 2021-04-13 DOI: 10.1038/s41596-021-00512-6

fetal imaging

micro-CT

postmortem

perinatal autopsy

high-resolution isotropic datasets

AI 解读

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Perinatal autopsy is the standard method for investigating fetal death; however, it requires dissection of the fetus. Human fetal microfocus computed tomography (micro-CT) provides a generally more acceptable and less invasive imaging alternative for bere
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
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References
ACKNOWLEDGMENTS

Vickerton, P., Jarvis, J. & Jeffery, N. Concentration-dependent specimen shrinkage in iodine-enhanced microCT. J. Anat. 223, 185–193 (2013).
Michalski, S. T., Porter, J. & Pauli, R. M. Costs and consequences of comprehensive stillbirth assessment. Am. J. Obstet. Gynecol. 186, 1027–1034 (2002).
MBRRACE-UK. Perinatal confidential enquiry: term, singleton, intrapartum stillbirth and intrapartum-related neonatal death. https://www.npeu.ox.ac.uk/assets/downloads/mbrrace-uk/reports/MBRRACE-UK%20Intrapartum%20Confidential%20Enquiry%20Report%202017%20-%20final%20version.pdf (2017).
Osborn, M., Lowe, J., Cox, P. G., Hargitai, B. & Marton, T. Royal College of Pathologists. Guidelines on autopsy practice: fetal autopsy (2nd trimester fetal loss and termination of pregnancy for congenital anomaly). https://www.rcpath.org/uploads/assets/b20ea503-7799-433c-99160653762f896c/Fetal-autopsy-2nd-trimester-fetal-loss-and-termination-of-pregnancy-for-congenital-anomaly.pdf (2017).
Blokker, B. M., Wagensveld, I. M., Weustink, A. C., Oosterhuis, J. W. & Hunink, M. G. Non-invasive or minimally invasive autopsy compared to conventional autopsy of suspected natural deaths in adults: a systematic review. Eur. Radiol. 26, 1159–1179 (2016).
Blokker, B. M. et al. Conventional autopsy versus minimally invasive autopsy with postmortem MRI, CT, and CT-guided biopsy: comparison of diagnostic performance. Radiology https://doi.org/10.1148/radiol.2018180924 (2018).
Lewis, C. et al. Factors affecting uptake of postmortem examination in the prenatal, perinatal and paediatric setting. BJOG 125, 172–181 (2018).
Osborn, M., Cox, P. G., Hargitai, B. & Marton, T. Royal College of Pathologists. Guidelines on autopsy practice: neonatal death. https://www.rcpath.org/uploads/assets/0a7c073e-c773-4941-a1e998df666e17e3/G168-Guidelines-on-autopsy-practice-Neonatal-death.pdf (2019).
Sieswerda-Hoogendoorn, T. & van Rijn, R. R. Current techniques in postmortem imaging with specific attention to paediatric applications. Pediatr. Radiol. 40, 141–152 (2010).
Royal College of Obstetricians and Gynaecologists & Royal College of Pathologists. Fetal and perinatal pathology: report of a working party. https://www.rcpath.org/uploads/assets/19f28c61-2a55-4eba-a3d9bf652a803424/FetalAndPerinatalPath-Jun01.pdf (2001).
Lewis, C. et al. Availability of less invasive prenatal, perinatal and paediatric autopsy will improve uptake rates: a mixed-methods study with bereaved parents. BJOG 126, 754 (2019).
Kang, X. et al. Parental acceptance of minimally invasive fetal and neonatal autopsy compared with conventional autopsy. Prenat. Diagn. 34, 1106–1110 (2014).
Taher, M. B., Pearson, J., Cohen, M. & Offiah, A. C. Acceptability of post-mortem imaging among Muslim and non-Muslim communities. Br. J. Radiol. 91, 20180295 (2018).
Lewis, C. et al. Minimally invasive autopsy for fetuses and children based on a combination of post-mortem MRI and endoscopic examination: a feasibility study. Health Technol. Assess. 23, 1–104 (2019).
Sonnemans, L. J. P. et al. Dutch guideline for clinical foetal-neonatal and paediatric post-mortem radiology, including a review of literature. Eur. J. Pediatr. 177, 791–803 (2018).
Arthurs, O. J., Taylor, A. M. & Sebire, N. J. Indications, advantages and limitations of perinatal postmortem imaging in clinical practice. Pediatr. Radiol. 45, 491–500 (2015).
Votino, C. et al. Virtual autopsy by computed tomographic angiography of the fetal heart: a feasibility study. Ultrasound Obstet. Gynecol. 39, 679–684 (2012).
Arthurs, O. J. et al. Diagnostic accuracy of post mortem MRI for abdominal abnormalities in foetuses and children. Eur. J. Radiol. 84, 474–481 (2015).
Arthurs, O. J. et al. Diagnostic accuracy of post-mortem MRI for thoracic abnormalities in fetuses and children. Eur. Radiol. 24, 2876–2884 (2014).
Arthurs, O. et al. Diagnostic accuracy and limitations of post-mortem MRI for neurological abnormalities in fetuses and children. Clin. Radiol. 70, 872–880 (2015).
Addison, S., Arthurs, O. J. & Thayyil, S. Post-mortem MRI as an alternative to non-forensic autopsy in foetuses and children: from research into clinical practice. Br. J Radiol. 87, 20130621 (2014).
Thayyil, S. et al. Post-mortem MRI versus conventional autopsy in fetuses and children: a prospective validation study. Lancet 382, 223–233 (2013).
Shelmerdine, S. C., Sebire, N. J. & Arthurs, O. J. Perinatal post mortem ultrasound (PMUS): a practical approach. Insights Imaging 10, 35 (2019).
Tuchtan, L. et al. Diagnosis of congenital abnormalities with post-mortem ultrasound in perinatal death. Diagn. Interv. Imaging 99, 143–149 (2018).
Shelmerdine, S. C., Sebire, N. J. & Arthurs, O. J. Perinatal post-mortem ultrasound (PMUS): radiological-pathological correlation. Insights Imaging 10, 81 (2019).
Shelmerdine, S. C., Hutchinson, J. C., Arthurs, O. J. & Sebire, N. J. Latest developments in post-mortem foetal imaging. Prenat. Diagn. 40, 28–37 (2020).
Kang, X., Carlin, A., Cannie, M., Sanchez, T. C. & Jani, J. C. Fetal postmortem imaging: an overview of current techniques and future perspectives. Am. J. Obstet. Gynecol, https://doi.org/10.1016/j.ajog.2020.04.034 (2020).
Jawad, N. et al. Body weight lower limits of fetal postmortem MRI at 1.5 T. Ultrasound Obstet. Gynecol. 48, 92–97 (2016).
Hutchinson, J. C. et al. Postmortem microfocus computed tomography for early gestation fetuses: a validation study against conventional autopsy. Am. J. Obstet. Gynecol. 218, 445.e441–445.e412 (2018).
Shelmerdine, S. C. et al. Postmortem microfocus computed tomography for noninvasive autopsies: experience in >250 human fetuses. Am. J. Obstet. Gynecol. https://doi.org/10.1016/j.ajog.2020.07.019 (2020).
Shelmerdine, S. C. et al. Characterization of Bardet–Biedl syndrome by postmortem microfocus computed tomography (micro-CT). Ultrasound Obstet. Gynecol. 53, 129–134 (2019).
Hutchinson, J. C. et al. Clinical utility of postmortem microcomputed tomography of the fetal heart: diagnostic imaging vs macroscopic dissection. Ultrasound Obstet. Gynecol. 47, 58–64 (2016).
Dawood, Y., Strijkers, G. J., Limpens, J., Oostra, R. J. & de Bakker, B. S. Novel imaging techniques to study postmortem human fetal anatomy: a systematic review on microfocus-CT and ultra-high-field MRI. Eur. Radiol. https://doi.org/10.1007/s00330-019-06543-8 (2019).
Eloot, L. et al. Quality control of micro-computed tomography systems. Radiat. Prot. Dosim. 139, 463–467 (2010).
Li, K. Z., Gao, Y., Zhang, R., Hu, T. & Guo, B. The effect of a manual instrumentation technique on five types of premolar root canal geometry assessed by microcomputed tomography and three-dimensional reconstruction. BMC Med. Imaging 11, 14 (2011).
Gregg, C. L. & Butcher, J. T. Quantitative in vivo imaging of embryonic development: opportunities and challenges. Differentiation 84, 149–162 (2012).
Aslanidi, O. V. et al. Application of micro-computed tomography with iodine staining to cardiac imaging, segmentation, and computational model development. IEEE Trans. Med. Imaging 32, 8–17 (2013).
Jacob, R. E. & Carson, J. P. Automated measurement of heterogeneity in CT images of healthy and diseased rat lungs using variogram analysis of an octree decomposition. BMC Med. Imaging 14, 1 (2014).
Al Faraj, A., Shaik, A. S. & Alnafea, M. Intrapulmonary administration of bone-marrow derived M1/M2 macrophages to enhance the resolution of LPS-induced lung inflammation: noninvasive monitoring using free-breathing MR and CT imaging protocols. BMC Med. Imaging 15, 16 (2015).
Chen, K. C., Arad, A., Song, Z. M. & Croaker, D. High-definition neural visualization of rodent brain using micro-CT scanning and non-local-means processing. BMC Med. Imaging 18, 38 (2018).
Thiboutot, J. et al. Current advances in COPD imaging. Acad. Radiol. https://doi.org/10.1016/j.acra.2018.05.023 (2018).
Sanchez, S., Fernandez, V., Pierce, S. E. & Tafforeau, P. Homogenization of sample absorption for the imaging of large and dense fossils with synchrotron microtomography. Nat. Protoc. 8, 1708–1717 (2013).
Kallai, I. et al. Microcomputed tomography-based structural analysis of various bone tissue regeneration models. Nat. Protoc. 6, 105–110 (2011).
Schambach, S. J., Bag, S., Schilling, L., Groden, C. & Brockmann, M. A. Application of micro-CT in small animal imaging. Methods 50, 2–13 (2010).
Arthurs, O. J. et al. Comparison of diagnostic performance for perinatal and paediatric post-mortem imaging: CT versus MRI. Eur. Radiol. 26, 2327–2336 (2016).
Norman, W., Jawad, N., Jones, R., Taylor, A. M. & Arthurs, O. J. Perinatal and paediatric post-mortem magnetic resonance imaging (PMMR): sequences and technique. Br. J. Radiol. 89, https://doi.org/10.1259/bjr.20151028 (2016).
Kang, X. et al. Post-mortem whole-body magnetic resonance imaging of human fetuses: a comparison of 3-T vs. 1.5-T MR imaging with classical autopsy. Eur. Radiol. 27, 3542–3553 (2017).
Staicu, A. et al. Potential clinical benefits and limitations of fetal virtopsy using high-field MRI at 7 Tesla versus stereomicroscopic autopsy to assess first trimester fetuses. Prenat. Diagn. 39, 505–518 (2019).
Thayyil, S. et al. Post-mortem examination of human fetuses: a comparison of whole-body high-field MRI at 9·4 T with conventional MRI and invasive autopsy. Lancet 374, 467–475 (2009).
Pauwels, E., Van Loo, D., Cornillie, P., Brabant, L. & Van Hoorebeke, L. An exploratory study of contrast agents for soft tissue visualization by means of high resolution X-ray computed tomography imaging. J. Microsc. 250, 21–31 (2013).
Dunmore-Buyze, P. J. et al. Three-dimensional imaging of the mouse heart and vasculature using micro-CT and whole-body perfusion of iodine or phosphotungstic acid. Contrast Media Mol. Imaging 9, 383–390 (2014).
Dullin, C. et al. muCT of ex-vivo stained mouse hearts and embryos enables a precise match between 3D virtual histology, classical histology and immunochemistry. PLoS ONE 12, e0170597 (2017).
Walton, L. A. et al. Morphological characterisation of unstained and intact tissue micro-architecture by X-ray computed micro- and nano-tomography. Sci. Rep. 5, 10074 (2015).
Gignac, P. M. & Kley, N. J. Iodine-enhanced micro-CT imaging: methodological refinements for the study of the soft-tissue anatomy of post-embryonic vertebrates. J Exp. Zool. B Mol. Dev. Evol. 322, 166–176 (2014).
Hopkins, T. M. et al. Combining micro-computed tomography with histology to analyze biomedical implants for peripheral nerve repair. J. Neurosci. Methods 255, 122–130 (2015).
Kim, A. J. et al. Microcomputed tomography provides high accuracy congenital heart disease diagnosis in neonatal and fetal mice. Circ. Cardiovasc. Imaging 6, 551–559 (2013).
Metscher, B. D. MicroCT for developmental biology: a versatile tool for high-contrast 3D imaging at histological resolutions. Dev. Dyn. 238, 632–640 (2009).
Metscher, B. D. MicroCT for comparative morphology: simple staining methods allow high-contrast 3D imaging of diverse non-mineralized animal tissues. BMC Physiol. 9, 11 (2009).
Degenhardt, K., Wright, A. C., Horng, D., Padmanabhan, A. & Epstein, J. A. Rapid 3D phenotyping of cardiovascular development in mouse embryos by micro-CT with iodine staining. Circ. Cardiovasc. Imaging 3, 314–322 (2010).
Lombardi, C. M. et al. Postmortem microcomputed tomography (micro-CT) of small fetuses and hearts. Ultrasound Obstet. Gynecol. 44, 600–609 (2014).
Sandaite, I. et al. Micro-computed tomography of isolated fetal hearts following termination of pregnancy: a feasibility study at 8–12 weeks’ gestation. Prenat. Diagn. https://doi.org/10.1002/pd.5719 (2020).
Sandrini, C. et al. Accuracy of micro-computed tomography in post-mortem evaluation of fetal congenital heart disease. Comparison between post-mortem Micro-CT and conventional autopsy. Front. Pediatr. 7, 92 (2019).
Hutchinson, J. C. et al. Virtual pathological examination of the human fetal kidney using micro-CT. Ultrasound Obstet. Gynecol. 48, 663–665 (2016).
Lombardi, S. et al. Micro-computed tomography: a new diagnostic tool in postmortem assessment of brain anatomy in small fetuses. Neuroradiology 61, 737–746 (2019).
Smith C. M. et al. HoloLens for medical imaging using post-mortem fetal micro-CT data. European Congress of Radiology Abstract. https://doi.org/10.26044/ecr2019/C-0153 (2019).
Shelmerdine, S. C. et al. 3D printing from microfocus computed tomography (micro-CT) in human specimens: education and future implications. Br. J. Radiol. https://doi.org/10.1259/bjr.20180306 (2018).

Human fetal whole-body postmortem microfocus computed tomographic imaging

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