Generation of heart-forming organoids from human pluripotent stem cells

Lika Drakhlis, Santoshi Biswanath Devadas, Robert Zweigerdt

Published: 2021-11-09 DOI: 10.1038/s41596-021-00629-8

human pluripotent stem cells

heart-forming organoids

WNT pathway modulators

protocol

lineage formation

AI 解读

Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells

The cell-line-derived subcutaneous tumor model in preclinical cancer research

Subcellular patch-clamp techniques for single-bouton stimulation and simultaneous pre- and postsynaptic recording at cortical synapses

NovoSpaRc: flexible spatial reconstruction of single-cell gene expression with optimal transport

Genome-wide pooled CRISPR screening in neurospheres

High-throughput cultivation and identification of bacteria from the plant root microbiota

Viral crosslinking and solid-phase purification enables discovery of ribonucleoprotein complexes on incoming RNA virus genomes

Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility

Addendum: Micro-CT acquisition and image processing to track and characterize pulmonary nodules in mice

Multiplexed single-cell proteomics using SCoPE2

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Heart-forming organoids (HFOs) derived from human pluripotent stem cells (hPSCs) are a complex, highly structured invitro model of early heart, foregut and vasculature development. The model represents a potent tool for various applications, including te
Introduction
Materials
Procedure
Troubleshooting
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Anticipated results
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References
ACKNOWLEDGMENTS
ACKNOWLEDGMENTS

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Generation of heart-forming organoids from human pluripotent stem cells

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