Generation of heart-forming organoids from human pluripotent stem cells

Lika Drakhlis, Santoshi Biswanath Devadas, Robert Zweigerdt

Published: 2021-11-09 DOI: 10.1038/s41596-021-00629-8

human pluripotent stem cells

heart-forming organoids

WNT pathway modulators

protocol

lineage formation

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Supplementary information

Supplementary Video 1

Movement of a T25 flask inside an incubator to ensure equal distribution of cells after seeding.

Supplementary Video 2

Embedding of an hPSC aggregate in a Matrigel droplet (d-2).

Supplementary Video 3

Removing the culture medium with 1000 µL and 200 µL tips.

Supplementary Video 4

Removing the culture medium with stacked tips (10 µL tip put on a 200 µL tip using a 20 – 200 µL pipet).

Supplementary Video 5

Transfer of a d10 HFO from the 96-U-well plate to a 12-well suspension plate using a 100 – 1000 µL pipet.

Fabrication of angstrom-scale two-dimensional channels for mass transport

Efficient and strand-specific profiling of replicating chromatin with enrichment and sequencing of protein-associated nascent DNA in mammalian cells

The eINTACT method for studying nuclear changes in host plant cells targeted by bacterial effectors in native infection contexts

Rapid reaction optimization by robust and economical quantitative benchtop19F NMR spectroscopy

Profiling native pulmonary basement membrane stiffness using atomic force microscopy

Photoimmunotechnology as a powerful biological tool for molecular-based elimination of target cells and microbes, including bacteria, fungi and viruses

Measuring key human carbohydrate digestive enzyme activities using high-performance anion-exchange chromatography with pulsed amperometric detection

Neural cell isolation from adult macaques for high-throughput analyses and neurosphere cultures

Preparation, validation and use of a vasoactive tryptophan-derived hydroperoxide and relevant control compounds

Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes

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Supplementary information

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Generation of heart-forming organoids from human pluripotent stem cells

Supplementary information

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