Cytosine and adenosine base editing in human pluripotent stem cells using transient reporters for editing enrichment

Stefan J. Tekel, Nicholas Brookhouser, Kylie Standage-Beier, Xiao Wang, David A. Brafman

Published: 2021-06-24 DOI: 10.1038/s41596-021-00552-y

human pluripotent stem cells

base editing

transient reporters

genomic editing

flow-cytometry

AI 解读

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Deaminase fused-Cas9 base editing technologies have enabled precise single-nucleotide genomic editing without the need for the introduction of damaging double-stranded breaks and inefficient homology-directed repair. However, current methods to isolate ba
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
- Reporting Summary
- References
References
ACKNOWLEDGMENTS

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Cytosine and adenosine base editing in human pluripotent stem cells using transient reporters for editing enrichment

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