Cytosine and adenosine base editing in human pluripotent stem cells using transient reporters for editing enrichment

Stefan J. Tekel, Nicholas Brookhouser, Kylie Standage-Beier, Xiao Wang, David A. Brafman

Published: 2021-06-24 DOI: 10.1038/s41596-021-00552-y

human pluripotent stem cells

base editing

transient reporters

genomic editing

flow-cytometry

AI 解读

Supplementary information

Reporting Summary

Inferring cellular and molecular processes in single-cell data with non-negative matrix factorization using Python, R and GenePattern Notebook implementations of CoGAPS

Dual detection of chromatin accessibility and DNA methylation using ATAC-Me

Single-particle tracking photoactivated localization microscopy of membrane proteins in living plant tissues

Design and simulation of DNA, RNA and hybrid protein–nucleic acid nanostructures with oxView

Stabilization and structure determination of integral membrane proteins by termini restraining

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Design, assembly, and characterization of membrane-spanning DNA nanopores

Hyperosmolar blood–brain barrier opening using intra-arterial injection of hyperosmotic mannitol in mice under real-time MRI guidance

Analysis framework and experimental design for evaluating synergy-driving gene expression

Medulloblastoma and high-grade glioma organoids for drug screening, lineage tracing, co-culture and in vivo assay

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Supplementary information

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Cytosine and adenosine base editing in human pluripotent stem cells using transient reporters for editing enrichment

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