Comparative analysis of assays to measure CAR T-cell-mediated cytotoxicity

Stefan Kiesgen, John C. Messinger, Navin K. Chintala, Zachary Tano, Prasad S. Adusumilli

Published: 2021-02-14 DOI: 10.1038/s41596-020-00467-0

CAR T-cell

cytotoxicity assay

immune effector cells

antitumor efficacy

FDA directives

AI 解读

Fabrication of angstrom-scale two-dimensional channels for mass transport

Efficient and strand-specific profiling of replicating chromatin with enrichment and sequencing of protein-associated nascent DNA in mammalian cells

The eINTACT method for studying nuclear changes in host plant cells targeted by bacterial effectors in native infection contexts

Rapid reaction optimization by robust and economical quantitative benchtop19F NMR spectroscopy

Profiling native pulmonary basement membrane stiffness using atomic force microscopy

Photoimmunotechnology as a powerful biological tool for molecular-based elimination of target cells and microbes, including bacteria, fungi and viruses

Measuring key human carbohydrate digestive enzyme activities using high-performance anion-exchange chromatography with pulsed amperometric detection

Neural cell isolation from adult macaques for high-throughput analyses and neurosphere cultures

Preparation, validation and use of a vasoactive tryptophan-derived hydroperoxide and relevant control compounds

Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes

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The antitumor efficacy of genetically engineered ‘living drugs’, including chimeric antigen receptor and T-cell receptor Tcells, is influenced by their activation, proliferation, inhibition, and exhaustion. A sensitive and reproducible cy
Main
51Cr assay
BLI luciferase assay
Impedance-based assay
Flow assay
Alternative assays
Advanced invitro antitumor efficacy assays
Potency assays for clinical cell therapy products
Assessment of cell therapies beyond cytotoxicity
Discussion
References
ACKNOWLEDGMENTS

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Comparative analysis of assays to measure CAR T-cell-mediated cytotoxicity

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