An end-to-end workflow for nondestructive 3D pathology

Adam K. Glaser, Jonathan T. C. Liu, Joshua C. Vaughan, Kevin W. Bishop, Lindsey A. Erion Barner, Qinghua Han, Elena Baraznenok, Lydia Lan, Chetan Poudel, Gan Gao, Robert B. Serafin, Sarah S. L. Chow, Andrew Janowczyk, David Brenes, Hongyi Huang, Dominie Miyasato, Lawrence D. True, Soyoung Kang

Published: 2024-01-23 DOI: 10.1038/s41596-023-00934-4

data processing

3D pathology

light-sheet microscopy

tissue preparation

fluorescent staining

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Supplementary information

Supplementary Information

Supplementary Notes 1–3 and Figs. 1–10

Reporting Summary

Supplementary Video

Video abstract, including volumetric renderings and 2D cross sections of Path3D datasets false-colored to mimic the appearance of standard H&E staining (human prostate biopsy and Barrett’s esophagus)

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An end-to-end workflow for nondestructive 3D pathology

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