Algae Agar Preparation
Melissa B Duhaime, Dylan J Baker, A.j Wing
Published: 2023-07-12 DOI: 10.17504/protocols.io.j8nlkewj6l5r/v1
Abstract
This protocol details the making of algae concentrate agar for the growth of bacteria from Douglas Lake.
Protocol for class project in Microbes in the Wild: Advanced Environmental Microbiology Lab (EEB 447) course at University of Michigan Biological Station and on campus.
Steps
1.
During the peak of a cyanobacterial bloom, bring a 100μm mesh out into the water. 1. Collect as many colonies as possible using the mesh and collect them into three sterile 50 ml falcon tubes.
2.
- Sterilize a conventional blender with 10% bleach. Rinse with 1-2L MilliQ water
- Pour the contents of the three 50 ml falcon tubes into the blender. Blend on high for 2 minutes.
- Add the contents of the blender into a sterile 1L round glass media bottle.
- Add 15 grams of agar to the media bottle.
- Bring up the mixture to 1L with MilliQ water
- Steam autoclave at 121˚C for 30 minutes on Liquid Cycle 2
- Pour agar into sterile petri dishes and allow to set. Store plates at 4˚C.
