Single-cell RNA-seq of maize meiocytes and pollen grains
Mary Washburn, Jaime Alaniz-Fabián, Taylor Scroggs, Brad Nelms
Supplementary information
Supplementary Information
Supplementary Figs. 1 and 2 and Supplementary Table 2
Supplementary Table
Supplementary Table 1. Sequence of barcoded CEL-seq primers. The first of these primers is shown in Supplementary Figure 2a with each section annotated. This table can be directly uploaded to IDT for ordering plate-based oligos. Because there are randomized bases in these primers (Ns), the automated form will ask if you want ‘machine-mixed’ or ‘hand-mixed’ bases during ordering; select machine-mixed bases.
Supplementary Data 1
Supplementary Data 1. CELseq_whitelist_legacy.txt. Legacy CELseq whitelist for Salmon Alevin. Use when re-analyzing data from ref. 3.
Supplementary Data 2
Supplementary Data 2. TGMAP.tsv. Transcript to gene mapping for Salmon Alevin.
Supplementary Data 3
Supplementary Data 3. CELseq_whitelist.txt. CELseq whitelist for Salmon Alevin. Use when analyzing new data generated with this protocol.
Supplementary Video 1
Supplementary Video 1. Technique to press anthers and release meiotic cells or pollen precursors (Step 5A(v–vii) and 5B(ii)).
Supplementary Video 2
Supplementary Video 2. Technique to isolate single cells and pollen precursors by using a syringe needle (Steps 6–12).
Supplementary Video 3
Supplementary Video 3. Handling of single-cell material on PCR caps (Steps 14 and 22–25).
