Spatial Transcriptomics for OCT using 10x Genomics Visium

Start here

If performing OCT tissue embedding for the first time be sure to consult pgs.5-9

If utilizing a new tissue type for the first time be sure to utilize the Tissue Optimization Guide which can be found here

RNA extraction and assessment is outlined in the above guide but not always necessary

Most OCT blocks have preserved RNA quality well due to their preservation/storage

Perform all fixation/H&E staining/imaging steps on the Visium Spatial Gene Expression Slide (PN-2000233)

Utilize the guide here

Typically it is not necessary to utilize a coverslip while imaging

Immediately proceed to the Visium Library Construction

Start on pg. 35 using this guide here

Sequence the libraries according to the requirements on pg. 59 of CG000239