Tutorial: methods for three-dimensional visualization of archival tissue material

Tariq Sami Haddad, Peter Friedl, Navid Farahani, Darren Treanor, Inti Zlobec, Iris Nagtegaal

Published: 2021-10-28 DOI: 10.1038/s41596-021-00611-4

sample preparation

three-dimensional visualization

archival tissue

microscopy techniques

disease mechanisms

AI 解读

Author Correction: Full-length sequencing of circular DNA viruses and extrachromosomal circular DNA using CIDER-Seq

Generation of heart-forming organoids from human pluripotent stem cells

Design and fabrication of wearable electronic textiles using twisted fiber-based threads

Activation of mechanoluminescent nanotransducers by focused ultrasound enables light delivery to deep-seated tissue in vivo

Chromatin accessibility profiling by ATAC-seq

Deoxyfluorination of phenols for chemoselective18F-labeling of peptides

Author Correction: cfSNV: a software tool for the sensitive detection of somatic mutations from cell-free DNA

Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids

An inexpensive semi-automated sample processing pipeline for cell-free RNA extraction

Application of CHyMErA Cas9-Cas12a combinatorial genome-editing platform for genetic interaction mapping and gene fragment deletion screening

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Analysis of three-dimensional patient specimens is gaining increasing relevance for understanding the principles of tissue structure as well as the biology and mechanisms underlying disease. New technologies are improving our ability to visualize large vo
Introduction
Serial methods for tissue processing
- 3D histology
- Knife-edge scanning microscopy
Nonserial methods for tissue processing
Data processing and analysis
Selection criteria
Concluding remarks
- References
References
ACKNOWLEDGMENTS

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Tutorial: methods for three-dimensional visualization of archival tissue material

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