Viral purification from faecal sample

Homogenise 200 mg of faecal sample in 30 ml of SM buffer (50 mM Tris-HCl pH 7.5, 100 mM NaCl, 8 mM MgSO4)

Centrifuge sample at 4000 rpm for 20 min, then filter through a 0.2 μm filter

Add filtrate to a 100-kDa-molecular-mass Amicon Ultra-15 Centrifugal Filter

Centrifuge filter tube at 4000 rpm for 3 min to concentrate sample

Repeat centrifugation until the entire diluted filtrate has been passed through the filter tube

Dilute filtrate with 30 ml SM buffer

Add diluted filtrate to a fresh 100-kDa-molecular-mass Amicon Ultra-15 Centrifugal Filter

Repeat centrifugation until a filtrate volume of at least 500 μl is reached

Add 10 U of DNase I and 10 U of RNase to the filtrate and incubate at 37°C for 30 min

Incubate samples at 65°C for 15 min to inactivate enzymes

Store at 4°C