TS Spurrs - unstained FFPE on Thermanox coverslips (TM - 013)

sandra.crameri

Published: 2023-05-09 DOI: 10.17504/protocols.io.3byl4b7rjvo5/v4

AI 解读

Abstract

Processing unstained FFPE sections on Thermanox coverslips to Spurrs resin block for EM.

Steps

SAN:

SPEC No:

OPERATOR & STEPS:

Dewax FFPE sections

or X3B 0h 5m 0s

Rehydrate

100% volume 0h 5m 0s

95% volume 0h 5m 0s

70% volume 0h 5m 0s

Conventional - with reduced times for 5um thick FFPE section

2.5%% volume in 0.1Molarity (M) Sorenson's phosphate buffer 07.2 300 mosmol/kg0h 20m 0s.

Wash buffer 0h 5m 0s

10.

1Mass / % volume in buffer0h 30m 0s

11.

70% volume 0h 10m 0s

12.

95% volume 0h 5m 0s

13.

100% volume 0h 5m 0s

14.

100% volume 0h 5m 0s

15.

100% volume Spurrs:100% volume in 1:1 ratio0h 30m 0s

16.

100% volume Spurrs0h 30m 0s

17.

100% volume Spurrs0h 30m 0s

18.

Oven polymerise 65°C

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TS Spurrs - unstained FFPE on Thermanox coverslips (TM - 013)

Abstract

Steps

HEADER

Dewax FFPE sections

Rehydrate

Conventional - with reduced times for 5um thick FFPE section

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