TS Spurrs - cell pellet (TM - 013, sections 5.3 & 6.6)
sandra.crameri
Published: 2023-05-07 DOI: 10.17504/protocols.io.81wgb6jpolpk/v1
Abstract
This method is used for conventional processing of cell pellets to Spurrs resin.
Steps
HEADER
1.
SAN:
SPEC No:
OPERATOR & STEPS:
OPERATOR & STEPS:
CONVENTIONAL
2.
2.5% volume 0h 40m 0s
3.
Wash 0.1Molarity (M) Sorenson's Phosphate Buffer 07.2 (300mosmol/kg) for 0h 15m 0s
4.
1% volume 1h 0m 0s
5.
70% volume 0h 20m 0s
6.
95% volume 0h 20m 0s
7.
100% volume 0h 20m 0s
8.
100% volume 0h 20m 0s
9.
50% volume 50% volume 0h 30m 0s
10.
100% volume 0h 30m 0s
11.
100% volume 1h 0m 0s
12.
Oven polymerise overnight at 65°C
