Stress-free single-cell transcriptomic profiling and functional genomics of murine eosinophils

Costanza Borrelli, Alessandra Gurtner, Isabelle C. Arnold, Andreas E. Moor

Published: 2024-03-19 DOI: 10.1038/s41596-024-00967-3

CRISPR screening

single-cell RNA sequencing

eosinophils

granulocytes

intestinal subsets

AI 解读

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Global analysis of RNA-binding protein dynamics by comparative and enhanced RNA interactome capture

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Sections

Figures

References

Introduction
Materials
- Stress-free scRNASeq of tissue resident eosinophils
- Pooled CRISPRi screen in bone-marrow derived eosinophils
Procedure 1: single-cell profiling of tissue resident eosinophils
Procedure 2: pooled CRISPRi screen in bone-marrow derived eosinophils
Troubleshooting
Timing
- Single-cell profiling of tissue resident eosinophils
- Pooled CRISPRi screen in bone marrow-derived eosinophils
Anticipated results
- Reporting summary
Acknowledgements

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Stress-free single-cell transcriptomic profiling and functional genomics of murine eosinophils

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