Starting a MinION sequencing run using MinKNOW

If required plug the MinION into the computer and wait for the MinION and flowcell to ben detected.

Choose flow cell 'FLO-MIN106' from the drop-down menu.

Then select the flowcell so a tick appears.

Click the 'New Experiment' button in the bottom left of the screen.

On the New experiment popup screen, select the running parameters for your experiment from the individual tabs:

Experiment: Name the run in the experiment field, leave the sample field blank.

Kit: Selection: Select LSK109 as there is no option for native barcoding (NBD104).

Run Options: Set the run length to 6 hours (you can stop the run once sufficient data has been collected as determined using RAMPART).

Basecalling: Leave basecalling turned but select 'fast basecalling'.

Output: The number of files that MinKNOW will write to a single folder. By default this is set to 4000 but can be reduced to make RAMPART update more frequently.

Click 'Start run'.

Monitor the progress of the run using the MinKNOW interface.