Size selection (Purification)
Tsu-Chun Hung
Abstract
Size selection (Purification)
Steps
Prepare 20µL sample and 9µL magnetic beads in 1.5 mL eppendorf tube.
| A | B | C | D |
|---|---|---|---|
| Component | Volume | Proportion | Note |
| Sample | 20 μl | 1x | |
| Magnetic beads | 9 μl | 0.45x | BeaverBeads™ DNA Select Isolation |
Mix sample and beads gently by flicking then flash spin the tube. Put on the regular rack for 5mins 0h 5m 0s.
Transfer the tube to the magnetic rack. After most of the magnetic beads attach to the wall, remove the supernant.
Add 300µL 75% ethanol, flip whole magnetic rack around. Wait for 3 mins 0h 3m 0s and remove the supernant.
Repeat step 4 .
Quick spin the tube, and put on the magnetic rack. Remove superfluous solution with 10 μl pipette.
Add 10µL elution buffer. Mix gently by flicking and flash spin the tube. Put on the regular rack for 10mins 0h 10m 0s.
Transfer the tube to the magnetic rack. After most of the magnetic beads attach to the wall, collect the supernant to 200 μl PCR tube or 8-strip PCR tube.
Ready for 2' PCR.
