Tutorial: guidelines for the computational analysis of single-cell RNA sequencing data Tallulah S. Andrews, Vladimir Yu Kiselev, Davis McCarthy, Martin Hemberg Published: 2020-12-06 DOI: 10.1038/s41596-020-00409-w computational analysis single-cell RNA sequencing workflow best practices tutorial AI 解读 浏览 1407 PDFMetadata 暂无数据 推荐阅读 Nature Protocols Protocols IO Current Protocols Author Correction: Full-length sequencing of circular DNA viruses and extrachromosomal circular DNA using CIDER-Seq Generation of heart-forming organoids from human pluripotent stem cells Design and fabrication of wearable electronic textiles using twisted fiber-based threads Activation of mechanoluminescent nanotransducers by focused ultrasound enables light delivery to deep-seated tissue in vivo Chromatin accessibility profiling by ATAC-seq Deoxyfluorination of phenols for chemoselective18F-labeling of peptides Author Correction: cfSNV: a software tool for the sensitive detection of somatic mutations from cell-free DNA Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids An inexpensive semi-automated sample processing pipeline for cell-free RNA extraction Application of CHyMErA Cas9-Cas12a combinatorial genome-editing platform for genetic interaction mapping and gene fragment deletion screening 查看全部 In-vitro GCase Activity Assay ASAPCRN GCase GCase activity Isolation of NeuN+ cells from brain tissue (for CUT and RUN) ASAPCRN URMC TriState SenNet Mouse Lung Digestion cellular senescence lung tissue Dissociation Long Amplicon Nanopore Sequencing for Dual-Typing RdRp and VP1 Genes of Norovirus Genogroups I and II in Wastewater Norovirus Oxford Nanopore Technologies Long Amplicon Wastewater Sequencing Assessment of prepulse inhibition (PPI) of the acoustic startle reflex in rodents Histology, Immunohistochemistry and Imaging ASAPCRN Differentiation NPCs to Dopaminergic/Midbrain Neurons differentiation dxn NPC Dopaminergic Midbrain Neurons ASAPCRN LRRK2-RCKW: MLi-2: E11 DARPin cryo-EM sample preparation ASAPCRN Tissue collection Characterizing spatial and temporal properties of ER-phagy receptors ASAPCRN 查看全部 Improving Species Level-taxonomic Assignment from 16S rRNA Sequencing Technologies 16S rRNA gene amplicons DADA2 microbiota composition metagenomics species-classification Efficient Isolation and Purification of High-Quality Arabidopsis thaliana Trichomes Arabidopsis thaliana density gradient centrifugation EDTA leaf trichome Generation of Bispecific Antibodies by Functionalized Poly-ADP-Ribose Polymers antibody bispecific antibody chemical conjugation immunotherapy PARP1 poly-ADP-ribose post-translational modification High-Throughput Gene Replacement in Aspergillus fumigatus Aspergillus fumigatus fusion PCR genome-wide knockout library high-throughput gene editing Human Umbilical Cord Mesenchymal Stem Cell–Based in vitro Model for Neurotoxicity Testing cytotoxicity test mesenchymal stem cells neuron-like cells neuronal markers Culture of Rat Primary Cortical Cells for Microelectrode Array (MEA) Recordings to Screen for Acute and Developmental Neurotoxicity acute subchronic and chronic exposure microelectrode array (MEA) recordings neuronal activity analysis neurotoxicity screening rat primary cortical cultures Hi-C 3.0: Improved Protocol for Genome-Wide Chromosome Conformation Capture chromatin interactions chromosome conformation capture cross-linking Hi-C restriction enzyme Fluorescent Protein Production, Purification, and Coupling to Microspheres fluorescent proteins full-spectrum cytometry microspheres protein expression protein purification ntEdit+Sealer: Efficient Targeted Error Resolution and Automated Finishing of Long-Read Genome Assemblies assembly finishing Bloom filter hybrid assembly polishing k-mer long-read genome assembly Chloroplast Transformation in Arabidopsis Arabidopsis biolistic transformation chloroplast transformation plastid transformation transplastomic plant 查看全部