Tutorial: guidelines for the computational analysis of single-cell RNA sequencing data Tallulah S. Andrews, Vladimir Yu Kiselev, Davis McCarthy, Martin Hemberg Published: 2020-12-06 DOI: 10.1038/s41596-020-00409-w computational analysis single-cell RNA sequencing workflow best practices tutorial AI 解读 浏览 2127 PDFMetadata 暂无数据 推荐阅读 Nature Protocols Protocols IO Current Protocols Designer DNA nanostructures for viral inhibition Tutorial: practical considerations for tissue clearing and imaging Surgical implantation of wireless, battery-free optoelectronic epidural implants for optogenetic manipulation of spinal cord circuits in mice In vivo and ex vivo electrophysiological study of the mouse heart to characterize the cardiac conduction system, including atrial and ventricular vulnerability Norovirus detection in water samples at the level of single virus copies per microliter using a smartphone-based fluorescence microscope Author Correction: Establishment of patient-derived cancer organoids for drug-screening applications Graphene- and metal-induced energy transfer for single-molecule imaging and live-cell nanoscopy with (sub)-nanometer axial resolution Design and simulation of DNA, RNA and hybrid protein–nucleic acid nanostructures with oxView Synthesis of68Ga-radiopharmaceuticals using both generator-derived and cyclotron-produced68Ga as exemplified by [68Ga]Ga-PSMA-11 for prostate cancer PET imaging Biotemplated precise assembly approach toward ultra-scaled high-performance electronics 查看全部 Quantification of TH immunoreactivity Quantification of TH immunoreactivity ASAPCRN Sanger Tree of Life RNA Extraction: Manual TRIzol™ RNA extraction manual RNA extraction TRIzol reference genome RNA-Seq IsoSeq Janelia Atalanta series plasmid cloning Time-lapse killing assay (monolayer - IncuCyte) ORFanID Web-based Search Engine to Identify Orphan and Taxonomically Restricted Genes ORFan genes ORFanID NCBI genomes Human Placenta Tissue Collection and Preservation Methods - UCSD Female Reproductive TMC HuBMAP UCSD Female Reproductive TMC Placenta Intracellular neuromelanin quantification Full plate & spot test plaque assays + PFU/mL calc. - anaerobic/slow growing bacteria Serotyping Crystallization of Zika NS5 RdRp crystallisation XChem ASAP AViDD CMD Diamond Light Source i04-1 Zika NS5 RdRp NS5 RdRp Research complex at Harwell 查看全部 Agrobacterium-Mediated Transformation of Setaria viridis, a Model System for Cereals and Bioenergy Crops AGL1 Agrobacterium-mediated transformation Agrobacterium tumefaciens bioenergy crops C4 photosynthesis cereals foxtail millet ME034V-1 model system Setaria viridis transformation Congenital Deafness and Recent Advances Towards Restoring Hearing Loss animal models congenital deafness gene therapy hearing loss Getting Started with LINCS Datasets and Tools bioinformatics disease drug discovery gene sets visualization signature analysis web application Visualization of the Skeletal Muscle Stem Cell Niche in Fiber Bundles microenvironment MuSCs satellite cells skeletal muscle stem cell niche quiescence Two Detailed Plaque Assay Protocols for the Quantification of Infectious SARS-CoV-2 SARS-CoV-2 COVID-19 COVID19 virus quantification plaque assay Prediction of Acute Hepatotoxicity With Human Pluripotent Stem Cell-Derived Hepatic Organoids cytochrome P450 drug testing hepatic organoid hepatotoxicity human pluripotent stem cell differentiation liver Generation and Functional Characterization of Monocytes and Macrophages Derived from Human Induced Pluripotent Stem Cells differentiation functional characterization induced pluripotent stem cells macrophages monocytes Establishment of Humanized Mice from Peripheral Blood Mononuclear Cells or Cord Blood CD34+ Hematopoietic Stem Cells for Immune-Oncology Studies Evaluating New Therapeutic Agents humanized mouse model immune oncology patient-derived xenograft (PDX) models tumor-infiltrating lymphocytes Design and Derivation of Multi-Reporter Pluripotent Stem Cell Lines via CRISPR/Cas9n-Mediated Homology-Directed Repair CRISPR/Cas9n human pluripotent stem cells reporter cell lines transfection of cells Ultrasound-Guided Approaches to Improve Orthotopic Mouse Xenograft Models for Hepatocellular Carcinoma doxorubicin echography echo-guided injection hepatocarcinoma Huh-7 orthotopic 查看全部