Tutorial: guidelines for the computational analysis of single-cell RNA sequencing data Tallulah S. Andrews, Vladimir Yu Kiselev, Davis McCarthy, Martin Hemberg Published: 2020-12-06 DOI: 10.1038/s41596-020-00409-w computational analysis single-cell RNA sequencing workflow best practices tutorial AI 解读 浏览 1765 PDFMetadata 暂无数据 推荐阅读 Nature Protocols Protocols IO Current Protocols Reliable detection of somatic mutations in solid tissues by laser-capture microdissection and low-input DNA sequencing Large-scale preparation of fluorescence multiplex host cell reactivation (FM-HCR) reporters Constructing and interpreting volcano plots and activity maps to navigate homogeneous catalyst landscapes The use of methanol as a C1 building block Yeast-based bioproduction of disulfide-rich peptides and their cyclization via asparaginyl endopeptidases The maternal serum metabolome by multisegment injection-capillary electrophoresis-mass spectrometry: a high-throughput platform and standardized data workflow for large-scale epidemiological studies 3D human induced pluripotent stem cell–derived bioengineered skeletal muscles for tissue, disease and therapy modeling Temporally resolved transcriptional recording inE. coliDNA using a Retro-Cascorder Constructing a cost-efficient, high-throughput and high-quality single-molecule localization microscope for super-resolution imaging Comprehensive multi-attribute method workflow for biotherapeutic characterization and current good manufacturing practices testing 查看全部 Quantitative analyses of the ultrastructural features of dopaminergic axon terminals. Protocol #1: Tissue preparation for electron microscopy Dopamine Axon terminal Striatum Tyrosine hydroxylase (TH) Transmission Electron Microscopy (TEM) Electron microscopy (EM) Substantia nigra pars compacta (SNc) Ventral Tegmental Area (VTA) Vesicle Synapse Ultrastructure Immuno-EM Immunogold ASAPCRN Fluorescence_activity_assay_Interlab_Study_PCC_6803 Exp_02 Synthesis of Carbon Dots using Citric acid (Solvent Free reaction) HuBMAP | GE/Vanderbilt MALDI IMS and Cell DIVE™ Modality Overview FACS screening to detect regulators of ROS ASAPCRN Structural prediction of VPS13C with AlphaFold2 UCSF ChimeraX VPS13C ASAPCRN AlphaFold SenNet Case Selection Protocol | JHU TMC pathology histology digitalpathology machinelearning ai Preparing Agarose Gel RNA Slide Preparation Protocol (FFPE) for nanostring DSP - GeoMx - Human Breast samples 查看全部 Acute Myeloid Leukemia Minimal Residual Disease Detection: The Difference from Normal Approach acute myeloid leukemia AML flow cytometry minimal residual disease MRD ezTrack—A Step-by-Step Guide to Behavior Tracking behavioral analysis fear conditioning freeze position tracking Ultrasound-Guided Approaches to Improve Orthotopic Mouse Xenograft Models for Hepatocellular Carcinoma doxorubicin echography echo-guided injection hepatocarcinoma Huh-7 orthotopic Visualization of the Skeletal Muscle Stem Cell Niche in Fiber Bundles microenvironment MuSCs satellite cells skeletal muscle stem cell niche quiescence Triplet-Primed PCR Assays for Accurate Screening of FMR1 CGG Repeat Expansion and Genotype Verification capillary electrophoresis FMR1 fragile X syndrome melting curve analysis screening triplet-primed PCR Isolation of Adult Mouse Cardiac Fibroblasts cardiac fibroblasts differentiation fibrosis flow cytometry heart failure single-cell Using Fluorescent GAP Indicators to Monitor ER Ca2+ aequorin Ca2+ calibration fluorescence GFP imaging Efficient Isolation and Purification of High-Quality Arabidopsis thaliana Trichomes Arabidopsis thaliana density gradient centrifugation EDTA leaf trichome An Updated Guide to Robust Statistical Methods in Neuroscience curvature heteroscedasticity non-normality outliers skewed distributions STARR-seq and UMI-STARR-seq: Assessing Enhancer Activities for Genome-Wide-, High-, and Low-Complexity Candidate Libraries cis-regulatory element enhancer functional genomics gene expression gene regulation massively parallel reporter assay MPRA STARR-seq transcriptional regulation UMI-STARR-seq 查看全部