STC-1 cell culture
Michael J Hurley
Published: 2023-04-02 DOI: 10.17504/protocols.io.5jyl8jky8g2w/v1
Abstract
Cell culture of STC-1 mouse enteroendocrine cells.
Steps
STC-1 cell culture
1.
Obtain Mouse ( Mus musculus ) neuroendocrine duodenal adenoma STC-1 cells (RRID:CVCL_J405) from the American Type Culture Collection (ATCC-CRL-3254™).
2.
Grow STC-1 cells in DMEM:F12 Ham with GlutaMax™ media supplemented with 10 % charcoal absorbed foetal bovine serum (LabTech), 1 mM sodium pyruvate, non-essential amino acids (100 mM) and penicillin (100 U/ml) streptomycin (100 mg/ml) on 12 well plates at 37°C under saturating humidity in a 5% CO2/95% air mixture.
3.
Subculture cells when 80-90 % confluent using Accutase™ and replate at 1:10 dilution for maintenance or at an appropriate density for experiments.
