Rotarod test
mariangela.massarocenere
Published: 2024-01-31 DOI: 10.17504/protocols.io.kqdg3xo81g25/v1
Abstract
Protocol to assess motor coordination and balance by using an accelerating rod
Steps
1.
Habituate the animal for 1 h to the testing room before the test
2.
Place the rat on the elevated rod (47 cm from the floor) rotating at low speed (4 rpm) for at least 30 seconds before the first session of the test
3.
Place the animal on an accelerating rod from 4 to 40 rpm in 300 s
4.
Repeat step 3 for 3 times with at least 5 min of rest between trials
4.1.
Record the time the rat fall from the rod (maximum 300 seconds)
5.
Clean the apparatus every time between animals and trials
6.
Repeat steps 4-5 for 4 times in two consecutive days (with an inter-session interval of 3.5 h)
10.
