Quantifying the LAMP1 positive puncta
Narayana Yadavalli, Shawn M. Ferguson
Published: 2023-07-08 DOI: 10.17504/protocols.io.14egn2pb6g5d/v1
Abstract
This is describes quantifying the LAMP1 positive puncta.
Attachments
Steps
Quantifying the LAMP1 positive puncta
1.
Select cells of interest with similar shapes and without saturation, and isolate single ROI.
2.
Apply auto threshold to total stacks of the images.
3.
Estimate the number of LAMP1 positive vesicles in each ROI by using “Analyze particle” in ImageJ/Fiji.
4.
Plot the results by calculating the mean number of LAMP1 positive puncta per each cell.
