Protocol collection: Phage DNA isolation and chemical analysis

Adair Borges

Published: 2022-12-17 DOI: 10.17504/protocols.io.e6nvwj6w2lmk/v1

浏览 1123

Abstract

Bacteriophages (phages) are viruses that infect bacteria. Some phages chemically modify their genomes to protect them from degradation by bacterial immune systems. We can detect phage genome modifications with mass spectrometry (MS) and high-performance liquid chromatography (HPLC) of phage nucleosides. This collection contains protocols for the entire process: phage growth and concentration, DNA extraction, and chemical analysis of phage nucleosides. We optimized these protocols using E. coli phage T4 and B. subtilis phage SPO1.

Steps

推荐阅读

Nature Protocols

Protocols IO

Current Protocols

Fabrication of angstrom-scale two-dimensional channels for mass transport

Efficient and strand-specific profiling of replicating chromatin with enrichment and sequencing of protein-associated nascent DNA in mammalian cells

The eINTACT method for studying nuclear changes in host plant cells targeted by bacterial effectors in native infection contexts

Rapid reaction optimization by robust and economical quantitative benchtop19F NMR spectroscopy

Profiling native pulmonary basement membrane stiffness using atomic force microscopy

Photoimmunotechnology as a powerful biological tool for molecular-based elimination of target cells and microbes, including bacteria, fungi and viruses

Measuring key human carbohydrate digestive enzyme activities using high-performance anion-exchange chromatography with pulsed amperometric detection

Neural cell isolation from adult macaques for high-throughput analyses and neurosphere cultures

Preparation, validation and use of a vasoactive tryptophan-derived hydroperoxide and relevant control compounds

Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes

查看全部

扫码咨询