Preparation of frozen nuclei for single-nucleus RNA seq

Kimberly Siletti, Sten Linnarsson

Published: 2022-10-11 DOI: 10.17504/protocols.io.eq2lyrdpgx9k/v2
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Abstract

This protocol describes our preparation of nuclei for single-nucleus RNA sequencing on the 10X Genomics Chromium system, starting from FAC-sorted frozen nuclei. 

Before start

Attachments

Single Nuclei Prep for 10X 2019 v2.pdf

Steps

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