Preparation of PEI for primary coating of MaxOne (neuronal cultures)

Prepare 100 mL of 1X borate buffer:

5mL

95mL

7% PEI stock solution :

1.4mL

8.6mL

Mix 0h 30m 0s using a magnetic stirrer until fully dissolved

(can be stored in 0.5 mL aliquots at -20°C for 1 month).

Where is it sterilized? -

final 0.07% PEI solution

0.5mL in

49.5mL

filter through a 0.22 μm filter unit before use!