Overview of scRNA-seq of Human Knee Meniscus
molmer, Martin Lotz
Published: 2022-08-24 DOI: 10.17504/protocols.io.dm6gpb7p5lzp/v1
Abstract
The overview provides sub-protocols for each step required to complete scRNA-seq from human knee meniscus.
Steps
1.
Tissue Acquisition
2.
Tissue Quality Control
3.
Single-Cell Isolation
4.
cDNA Library Preparation
5.
Data Processing and Quality Control