Organoid Drug Treatment
yzhu
Published: 2024-06-14 DOI: 10.17504/protocols.io.x54v92rb1l3e/v1
Abstract
Protocol for drug experiments of organoid samples
Steps
1.
Trypsinize organoids and plate at 30,000 cells/well in 24-well plates
2.
Incubate 5-7 days
3.
Incubate organoids in a complete medium containing 500 nM JQ1 for 24 hours (JQ cells) or 0.1% v/v DMSO (control cells)
4.
Harvest organoids in Cell Recovery Solution (Corning) on ice for 1 hour, wash with PBS, and centrifuge to retrieve cell pellets
5.
Proceed to process cell pellets for RNA extraction
