Obtain LB agar plates (with antibiotics)
An.Huang
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Abstract
LB plate is a fundamental laboratory consumable. This protocol helps make basic LB agar plates.
Steps
Weigh 40g LB nutrient agar powder.
Dissolve the powder in 1000mL distilled water.
Autoclave the melting LB agar at 121°C for 0h 15m 0s.
When autoclaving procedure is finished, wait until the flask cools to around 40°C, which will not hurt the palm.
(Optional) If the plate is required to contain a certain antibiotic, add a certain amount of antibiotics.
Here ampicillin is used as an example:
Add 1mL 1000X ampicillin stock solution into 1000mL melting LB agar. Mix well.
Pour melting agar into empty petri dishes until the melting agar has completely cover the bottom of the petri dishes. Avoid bubbles.
Leave the plates to cool and the lids ajar.
Wait until all agar plates solidified. Invert the plates and store at 4°C.
