Nuclei Isolation for Human Ovary Explants

Nicolas Martin

Published: 2024-04-06 DOI: 10.17504/protocols.io.e6nvw1632lmk/v1

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This protocol needs prior approval by the users' institutional review board (IRB) or equivalent ethics committee(s).

Abstract

This protocol uses fresh frozen human ovary explants to isolate nuclei suspension.

Steps

Nuclei Isolation Protocol for Human Ovary Explants

Chapter 1—Single Cell Gene Expression & Chromium Fixed RNA Profiling of the protocol CG000505 REV A was used to isolate nuclei from frozen human ovary explants with the following modifications: 1) a cordless motor pestle (VWR, Catalog number 47747-370) was used for Step f, Page 30 and 2) the samples were incubated for 15 min on ice for Step h, Page 30.

https://www.10xgenomics.com/support/single-cell-gene-expression/documentation/steps/sample-prep/chromium-nuclei-isolation-kit-sample-prep-user-guide

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