NGM Agar

Adrien Assie, Buck Samuel

Published: 2021-10-01 DOI: 10.17504/protocols.io.zy7f7zn

C. elegans

Growing media

Caenorhabditis

nematode

浏览 1202

Disclaimer

This is a standard protocol for C. elegans maintenance available on wormbook.

Abstract

NGM agar recipe from wormbook

Steps

1.

Start with 975mL

2.

3.0g NaCl

3.

2.5gPeptone

4.

17gAgar

5.

Autoclave with stir bar

6.

Cool to 55°C (faster in water bath)

7.

Then add the following while stirring on heat plate (DO NOT OVERHEAT)

8.

0.5mL of 1Molarity (M) CaCl2 (sterile)

9.

1mLof 5 mg/mL Cholesterol (dissolved in ethanol)

10.

1mL of 1Molarity (M) MgSO4 (sterile)

11.

25mL of 1Molarity (M) Potassium Phosphate Buffer, pH 6.0 (sterile)

12.

Pour into petri dishes using sterile technique

60 mm dishes = About 7mL per plate
100 mm dishes = About 20mL per plate

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