NCEM Drop - Tissue Dounce Homogenisation (TM-014)

SAN:

SPEC No:

OPERATOR:

This method is often used for scabs, warts, or chunks of tissue such as liver. Place ~ 5mm x 5mm piece of scab or tissue into glass homogeniser tube.

Insert teflon plunger into cordless drill, set drill on max speed.

Add equal volume TC water to tube, homogenise(20 strokes) inside Class II BSC cabinet.

Clarify resultant solution at 13000rpm,0h 0m 0s in Eppendorf centrifuge for 0h 1m 0s

Stand for 0h 5m 0s to permit viruses to diffuse back into solution, from the debris, and provide an interface for the sampling of membrane associated viruses. Use supernatant for sample below.

Adsorb 10µL sample to grid 0h 10m 0s , inspect to ensure sample does not dry out.

Drain excess sample from grid using filter paper, leave wet.

Stain 0h 1m 0s

Drain & dry using filter paper