NCEM Drop - Inactivation of sample on grid (TM-014)

sandra.crameri

Published: 2023-05-13 DOI: 10.17504/protocols.io.8epv59mr6g1b/v1
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Abstract

NCEM Drop - inactivation of samples on grid

Steps

1.

Adsorb 10µL sample to grid 0h 10m 0s

2.

Immerse grid in drop of 2.5% volume in 0.1Molarity (M) Sorenson's phosphate buffer 0h 10m 0s

3.

Wash 0.1Molarity (M) Sorenson's phosphate buffer

4.

Using filter paper wick excess buffer from grid - do not dry out, leave grid surface wet.

5.

Stain 0h 1m 0s

6.

Using filter paper gradually wick stain dry from grid

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