Modified Dixon’s Agar (Heitman Lab)

Amy Gladfelter, Heitman Lab

Published: 2023-06-02 DOI: 10.17504/protocols.io.q26g7rp11vwz/v1
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Abstract

Media recipe for growth of Malassezia species.

Before start

Attachments

Malassezia media_1.pdf

Steps

1.

Malt extract . 36g

2.

Mycological peptone (Oxoid L40) 10g

3.

Dessicated ox bile (Oxoid) . 10g

4.

Agar 20g

5.

Tween 60 (heated before use) 10mL

6.

Glycerol, 50% 4mL

7.

dH2O 986mL

8.

Sterilize by autoclaving at 121°C for 0h 15m 0s.

9.

Cool to 50°C.

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