Making tetracycline LB agar plates

Autoclave or microwave LB agar from media room

Make up 15 mg/ml tetracycline hydrochloride in sterile water

Retrieve tetracycline hydrochloride from -20 °C freezer

Weigh an empty Eppendorf

In the fume hood, add tetracycline hydrochloride to the Eppendorf

Weigh the Eppendorf, and calculate the difference to determine the weight (mg) of compound

To calculate sterile water (uL) to add = (compound mg / 15 mg/mL) x 1000 uL

Let LB agar cool to 55 °C

Dilute tetracycline hydrochloride 1/1000 dilution in LB agar (final conc: 15 ug/mL).

Using a serological pipette, dispense 35 mL agar into 90 mm Petri dishes. Leave to set.