JMN-MSMP Muscle Bulk RNAseq
ccherry
Published: 2023-09-27 DOI: 10.17504/protocols.io.rm7vzxjz4gx1/v2
Abstract
RNA sequencing of muscle
Steps
Bulk RNA sequencing
1.
cDNA synthesis and library preparation were performed using the TruSeq RNA Library Prep Kit V2 following the manufacturer's protocol.
2.
The Agilent BioAnalyzer system was used to perform quality control on samples during the library prep process as suggested in the manufacturer's protocol and RNA samples with RIN scores below 7 post-cDNA synthesis were excluded from analysis.
3.
Libraries were pooled and sequenced to a target depth of 30M unique paired end 50bp x 50bp reads per sample using a NovaSeq 6000.
