Intraperitoneal L-dopa injection in mice
natalia.lopezgonzalezdelrey, Zachary Gaertner
Published: 2023-06-17 DOI: 10.17504/protocols.io.81wgbxd51lpk/v1
Abstract
Intraperitoneal L-dopa injection in mice
Steps
Preparation
1.
Mice were acclimated to the room for 1h 0m 0s
L-dopa preparation
2.
Prepare the L-dopa mix fresh every day.
Prepare a master mix of: 12.5mg/kg L-dopa and 12.5mg/kg benserazide dissolved in sterile saline with 0.25% citrate (wt/vol). Calculate grams depending on number of mice to be injected. Using vortex will dissolve the solution easier.
L-dopa injection
3.
Mice are injected intraperitoneally with the 12.5mg/kg L-dopa and 12.5mg/kg benserazide solution.
Behaviour
4.
One hour post injection, mice were tested using the appropiate behavior protocol.
