Implantation of Neuropixels probes for chronic recording of neuronal activity in freely behaving mice and rats

Rik J. J. van Daal, Çağatay Aydin, Frédéric Michon, Arno A. A. Aarts, Michael Kraft, Fabian Kloosterman, Sebastian Haesler

Published: 2021-06-08 DOI: 10.1038/s41596-021-00539-9

Neuropixels probes

behaving mice

chronic recording

3D-printed fixtures

single-unit data

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How dynamic activity in neural circuits gives rise to behavior is a major area of interest in neuroscience. A key experimental approach for addressing this question involves measuring extracellular neuronal activity in awake, behaving animals. Recently de
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ACKNOWLEDGMENTS

Jun, J. J. et al. Fully integrated silicon probes for high-density recording of neural activity. Nature 551, 232–236 (2017).
Steinmetz, N. A., Zatka-Haas, P., Carandini, M. & Harris, K. D. Distributed coding of choice, action and engagement across the mouse brain. Nature 576, 266–273 (2019).
Hartley, T., Lever, C., Burgess, N. & O’Keefe, J. Space in the brain: how the hippocampal formation supports spatial cognition. Philos. Trans. R. Soc. B Biol. Sci. 369, 20120510 (2014).
Krupic, J., Bauza, M., Burton, S. & O’Keefe, J. Local transformations of the hippocampal cognitive map. Science 359, 1143–1146 (2018).
Steinmetz, N. A. et al. Neuropixels 2.0: a miniaturized high-density probe for stable, long-term brain recordings. Science 372, eabf4588 (2021).
Berényi, A. et al. Large-scale, high-density (up to 512 channels) recording of local circuits in behaving animals. J. Neurophysiol. 111, 1132–1149 (2014).
Chung, J., Sharif, F., Jung, D., Kim, S. & Royer, S. Micro-drive and headgear for chronic implant and recovery of optoelectronic probes. Sci. Rep. 7, 2773 (2017).
Michon, F. et al. Integration of silicon-based neural probes and micro-drive arrays for chronic recording of large populations of neurons in behaving animals. J. Neural Eng. 13, 046018 (2016).
Van Daal, R. J. J. et al. System for recording from multiple flexible polyimide neural probes in freely behaving animals. J. Neural Eng. 17, 016046 (2020).
Juavinett, A. L., Bekheet, G. & Churchland, A. K. Chronically implanted Neuropixels probes enable high-yield recordings in freely moving mice. eLife https://doi.org/10.7554/eLife.47188 (2019).
Cohen, J. Y., Haesler, S., Vong, L., Lowell, B. B. & Uchida, N. Neuron-type-specific signals for reward and punishment in the ventral tegmental area. Nature 482, 85–88 (2012).
Krynitsky, J. et al. Rodent Arena Tracker (RAT): a machine vision rodent tracking camera and closed loop control system. eNeuro 7, 1–9 (2020).
Siegle, J. H. et al. Open Ephys: an open-source, plugin-based platform for multichannel electrophysiology. J. Neural Eng. 14, 045003 (2017).
Jun, J. J. et al. Real-time spike sorting platform for high-density extracellular probes with ground-truth validation and drift correction. Preprint at bioRxiv https://doi.org/10.1101/101030 (2017).
Pachitariu, M., Steinmetz, N., Kadir, S., Carandini, M. & Harris, K. Fast and accurate spike sorting of high-channel count probes with KiloSort. in Advances in Neural Information Processing Systems 29 (NeurIPS 2016).
Esquivelzeta Rabell, J., Mutlu, K., Noutel, J., Martin del Olmo, P. & Haesler, S. Spontaneous rapid odor source localization behavior requires interhemispheric communication. Curr. Biol. 27, 1542–1548.e4 (2017).

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Neuropixels probes

behaving mice

chronic recording

3D-printed fixtures

single-unit data

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