IPTG_induction
Ana Belem García González, Georgina Diego, Jair Alexis Gardea Sáenz, Irán Alessandra Chaparro Rodríguez
Published: 2022-10-09 DOI: 10.17504/protocols.io.4r3l2od1pv1y/v1
Abstract
Protocol to induce protein expression using IPTG.
Steps
Protocol
1.
From a relatively fresh plate, grab a colony and grow at 37°C and225rpm in 10mL of LB+CAM.
2.
Inoculate 3mL in 30mL of LB+CAM and incubate at 37°C and 225rpm
3.
When the cells reach to a OD600 between 0.4 and 0.8, the protein expression must be induced (IPTG concentrations can vary from 0.1 to 1M).
4.
Incubate a 37°Cat 225rpmfor 5h 0m 0s (Hours can vary).
