Genetic manipulation of giant viruses and their host,Acanthamoeba castellanii Nadege Philippe, Avi Shukla, Chantal Abergel, Hugo Bisio Published: 2023-11-14 DOI: 10.1038/s41596-023-00910-y CRISPR-Cas9 Genetic manipulation Giant viruses Acanthamoeba castellanii Homologous recombination AI 解读 浏览 1503 PDFMetadataSupplementary information Supplementary Information Supplementary Methods Reporting Summary 推荐阅读 Nature Protocols Protocols IO Current Protocols A dynamic DNA tetrahedron framework for active targeting An MSstats workflow for detecting differentially abundant proteins in large-scale data-independent acquisition mass spectrometry experiments with FragPipe processing BridGE: a pathway-based analysis tool for detecting genetic interactions from GWAS Tutorial: a guide for the selection of fast and accurate computational tools for the prediction of intrinsic disorder in proteins Structural characterization of protein-material interfacial interactions using lysine reactivity profiling-mass spectrometry TAC–TIC, a high-throughput genetics method to identify triggers or blockers of bacterial toxin–antitoxin systems Microbiology techniques Phage biology Prokaryote Bacteriophages Bacterial genetics Microtiter plate assays to assess antibiofilm activity against bacteria Engineering megabase-sized genomic deletions with MACHETE (Molecular Alteration of Chromosomes with Engineered Tandem Elements) A practical guide to large-scale docking Spatiotemporal control of RNA metabolism and CRISPR–Cas functions using engineered photoswitchable RNA-binding proteins 查看全部 Pilot Exercise: Generating the Illumina SampleSheet and sharing data via BaseSpace Immunohistochemical labelling of lower urinary tract afferents in spinal cord activity-mapping neuroanatomy immunohistochemistry MIBI staining SRA and Genbank BioSample-Linked Submission with Mercury_Prep and Mercury_Batch Vu, M-A. T. et al (2024) Targeted micro-fiber arrays for measuring and manipulating localized multi-scale neural dynamics over large, deep brain volumes during behavior Immunoblot S1: Step-by-step-guide using image J in the work flow. Hafting tests on tanged blanks archeology experimentation archeozoology lithic knapping hafting balistics use-wear MSA Cell culture Automated concentration of viral particles in wastewater samples using Nanotrap Microbiome A Beads Nanotrap automated concentration wastewater concentration SARS-CoV-2 查看全部 ntEdit+Sealer: Efficient Targeted Error Resolution and Automated Finishing of Long-Read Genome Assemblies assembly finishing Bloom filter hybrid assembly polishing k-mer long-read genome assembly Expansion Microscopy for Beginners: Visualizing Microtubules in Expanded Cultured HeLa Cells antibody beginner clearing expansion microscopy fluorescence microscopy HeLa cells hydrogel imaging immunocytochemistry immunohistochemistry microscopy microtubules super-resolution microscopy Testing Graphical Causal Models Using the R Package “dagitty” dagitty directed acyclic graphs (DAGs) independence testing model testing Animal Models of Osteoarthritis Part 1–Preclinical Small Animal Models: Challenges and Opportunities for Drug Development drug development guinea pig mouse osteoarthritis preclinical animal model rabbit rat zebrafish Coated Latex Beads as Artificial Cells for Quantitative Investigations of Receptor/Ligand Interactions beads flow cytometry quantification stimulation Functional Assays Evaluating Immunosuppression Mediated by Myeloid-Derived Suppressor Cells cell culture chronic inflammation flow cytometry immunosuppression inflammatory mouse model MDSC myeloid-derived suppressor cell Role of High-Throughput Electrophysiology in Drug Discovery drug discovery high-throughput electrophysiology ion channel patch clamp Differentiation of Human Induced Pluripotent Stem Cells into Keratinocytes epidermal biology induced pluripotent stem cell iPSC iPSC-derived keratinocytes keratinocytes skin diseases Downregulation of Hypoxia Inducible Factor-1α in Primary Human Natural Killer Cells Using Small Interfering RNA Delivery with ExPERT ATx by MaxCyte ExPERT ATx HIF-1α knockdown MaxCyte NK cells siRNA transfection mOTUs: Profiling Taxonomic Composition, Transcriptional Activity and Strain Populations of Microbial Communities metagenomics metatranscriptomics taxonomic profiling 查看全部